Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Protection of the rat liver against rewarming ischemic injury by University of Wisconsin solution
Autore:
Lutterova, M; Kukan, R; Vajdova, K; Kuba, D; Mislanova, C; Kebis, A; Danninger, F; Mraz, P;
Indirizzi:
Inst Prevent & Clin Med, Lab Perfused Organs, Bratislava 83301, Slovakia Inst Prevent & Clin Med Bratislava Slovakia 83301 islava 83301, Slovakia Derers Hosp, Dept Surg, Bratislava, Slovakia Derers Hosp Bratislava Slovakia s Hosp, Dept Surg, Bratislava, Slovakia
Titolo Testata:
LANGENBECKS ARCHIVES OF SURGERY
fascicolo: 1, volume: 386, anno: 2001,
pagine: 31 - 37
SICI:
1435-2443(200102)386:1<31:POTRLA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYPOTHERMIC PERFUSION; REPERFUSION INJURY; HEPATIC ISCHEMIA; BILE-FLOW; PRESERVATION; TRANSPLANTATION; HEPATECTOMY; VIABILITY; BLOCKADE;
Keywords:
ischemia/reperfusion; rat liver; TNF-alpha; adenine nucleotide degradation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Kukan, R Inst Prevent & Clin Med, Lab Perfused Organs, Limbova 14, Bratislava 83301, Slovakia Inst Prevent & Clin Med Limbova 14 Bratislava Slovakia83301 kia
Citazione:
M. Lutterova et al., "Protection of the rat liver against rewarming ischemic injury by University of Wisconsin solution", LANG ARCH S, 386(1), 2001, pp. 31-37

Abstract

Background/aim: University of Wisconsin (UW) solution has been proven ableto prevent liver injury during cold ischemia. During rewarming ischemia, however, the efficacy of this solution in preserving hepatocyte function is unclear. The aim of the present study was to investigate to what extent UW solution protects rat liver during rewarming ischemia. Methods: Livers werewashed out with cool physiologic saline or with UW solution and subjected to rewarming ischemia for periods of 20 min or 45 min followed by reperfusion using a blood-free perfusion model. Results: In comparison with controls, ischemia for 20 min in saline-treated livers led to mild depression of hepatocyte function, while UW solution afforded complete protection of the liver. In UW-treated livers, compared with saline-treated livers exposed to ischemia for 45 min, portal flow was slightly but significantly higher, bileproduction was increased by 62%, and lactate dehydrogenase leakage into the perfusate was reduced by 61%. In an attempt to explain mechanisms of liver protection by UW solution, we found that UW solution inhibited conversionof hypoxanthine into uric acid, but this effect was not associated with decreased degradation of adenine nucleotides in the liver during ischemia. Following 30 min reperfusion, UW solution increased tissue levels of adenosine triphosphate (not significantly) and adenosine diphosphate (significantly). Further, UW solution markedly reduced tumor necrosis factor-alpha release by the liver both after ischemia and after reperfusion. Conclusions: These results create the hypothesis that UW solution may protect liver tissue during ischemia in liver surgery as well as during the implantation stage ofliver transplantation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 09:31:20