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Titolo:
Improved adenovirus vectors for infection of cardiovascular tissues
Autore:
Havenga, MJE; Lemckert, AAC; Grimbergen, JM; Vogels, R; Huisman, LGM; Valerio, D; Bout, A; Quax, PHA;
Indirizzi:
Crucell Holland BV, NL-2301 CA Leiden, Netherlands Crucell Holland BV Leiden Netherlands NL-2301 CA CA Leiden, Netherlands TNO, PG, Gaubius Lab, NL-2301 CE Leiden, Netherlands TNO Leiden Netherlands NL-2301 CE us Lab, NL-2301 CE Leiden, Netherlands
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 7, volume: 75, anno: 2001,
pagine: 3335 - 3342
SICI:
0022-538X(200104)75:7<3335:IAVFIO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDE SYNTHASE GENE; IN-VITRO; ATHEROSCLEROTIC MONKEYS; CELL-PROLIFERATION; NONHUMAN-PRIMATES; RECEPTOR PROTEIN; EXPRESSION; VIVO; COXSACKIEVIRUS; GENERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Havenga, MJE Crucell Holland BV, POB 2048, NL-2301 CA Leiden, Netherlands Crucell Holland BV POB 2048 Leiden Netherlands NL-2301 CA ds
Citazione:
M.J.E. Havenga et al., "Improved adenovirus vectors for infection of cardiovascular tissues", J VIROLOGY, 75(7), 2001, pp. 3335-3342

Abstract

To identify improved adenovirus vectors for cardiovascular gene therapy, alibrary of adenovirus vectors based on adenovirus serotype 5 (Ad5) but carrying fiber molecules of other human serotypes, was generated. This librarywas tested for efficiency of infection of human primary vascular endothelial cells (ECs) and smooth muscle cells (SMCs). Based on luciferase, LacZ, or green fluorescent protein (GFP) marker gene expression, several fiber chimeric vectors were identified that displayed improved infection of these cell types. One of the viruses that performed particularly well is an Ad5 carrying the fiber of Ad16 (Ad5.Fib16), a subgroup B virus. This virus showed,on average, 8- and 64-fold-increased luciferase activities on umbilical vein ECs and SMCs, respectively, compared to the parent vector. GFP and lacZ markers showed that approximately 3-fold (ECs) and 10-fold (SMCs) more cells were transduced. Experiments performed with both cultured SMCs and organ cultures derived from different vascular origins (saphenous vein, iliac artery, left interior mammary artery, and aorta) and from different species demonstrated that Ad5.Fib16 consistently displays improved infection in primates (humans and rhesus monkeys). SMCs of the same vessels of rodents and pigs were less infectable with Ad5.Fib16 than with Ad5. This suggests that either the receptor for human Ad16 is not conserved between different speciesor that differences in the expression levels of the putative receptor exist. In conclusion, our results show that an Ad5-based virus carrying the fiber of Ad16 is a potent vector for the transduction of primate cardiovascular cells and tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 04:24:05