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Titolo:
Specific amino acids moderate the effects of Ni2+ on the testosterone production of mouse Leydig cells in vitro
Autore:
Forgacs, Z; Nemethy, Z; Revesz, C; Lazar, P;
Indirizzi:
Jozsef Fodor Natl Ctr Publ Hlth, Natl Inst Chem Safety, Dept Mol & Cell Biol, H-1450 Budapest, Hungary Jozsef Fodor Natl Ctr Publ Hlth Budapest Hungary H-1450 udapest, Hungary Hungarian Acad Sci, Inst Expt Med, Budapest, Hungary Hungarian Acad Sci Budapest Hungary i, Inst Expt Med, Budapest, Hungary
Titolo Testata:
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
fascicolo: 5, volume: 62, anno: 2001,
pagine: 349 - 358
SICI:
1528-7394(20010309)62:5<349:SAAMTE>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
NICKEL COMPOUNDS; TOXICITY; CARCINOGENICITY; ANTAGONISM; CULTURE; CADMIUM; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Forgacs, Z Jozsef Fodor Natl Ctr Publ Hlth, Natl Inst Chem Safety, Dept Mol & Cell Biol, POB 36, H-1450 Budapest, Hungary Jozsef Fodor Natl Ctr Publ Hlth POB 36 Budapest Hungary H-1450
Citazione:
Z. Forgacs et al., "Specific amino acids moderate the effects of Ni2+ on the testosterone production of mouse Leydig cells in vitro", J TOX E H A, 62(5), 2001, pp. 349-358

Abstract

The purpose of this investigation was to study the effectiveness of two nickel-binding amino acids, histidine (His) and cysteine (Cys), to prevent the inhibitory action of Ni2+ on testosterone (T) production by mouse primaryLeydig cell culture. The maximal human chorionic gonadotropin (hCG)-stimulated T response was measured by radioimmunoassay (RIA) in the culture media. Three types of experiments were performed. In a concentration-response study, Ni2+ (62.5 to 1000 muM) was added to the cells simultaneously with equimolar or twice the equimolar concentrations of His or Cys and the cultureswere maintained for 48 h. Nickel-induced reduction in T production was completely prevented by equimolar concentrations of His at Ni2+ concentrationsof 125, 250, and 500 muM; equimolar or twice the equimolar concentrations of His were only partially effective at 1000 muM Ni2+. Protective action ofCys was complete only at the lowest concentration of Ni2+ (125 muM). In a second series, the cells were incubated for various times (0.5 to 48 h) with 1000 muM Ni2+ in the presence of 2000 muM His or Cys. Increasing the timeof incubation, the protective effect of both amino acids against Ni2+ was reduced. In a third series, attempts were made to reverse the action of 1000 muM Ni2+ after incubation with cells for various times (0.5 to 24 h), followed by exposure to 2000 muM His or Cys. Cell cultures were maintained for48 h. A partial recovery of hCG-stimulated T production could be observed only if the amino acid was added not later than 4 h after the metal. This time was also required to elicit the T depression produced by Ni'(2+). Administration of either His or Cys at later times had no effect. Our results show that both His and Cys are able to moderate the effects of Ni2+ on Leydigcell T production, depending on the concentration of this metal ion, as well as on amino acid. However, at higher Ni2+ concentrations the complete protection by His or Cys is only temporary. Administration of these amino acids after the Ni2+-produced decrease in T production war notable to reverse the process.

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Documento generato il 28/01/20 alle ore 20:57:05