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Titolo:
The neuronal norepinephrine transporter in experimental heart failure: Evidence for a posttranscriptional downregulation
Autore:
Backs, J; Haunstetter, A; Gerber, SH; Metz, J; Borst, MM; Strasser, RH; Kubler, W; Haass, M;
Indirizzi:
Univ Heidelberg, Dept Cardiol, D-69115 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-69115 , D-69115 Heidelberg, Germany Dept Anat & Cell Biol, D-69120 Heidelberg, Germany Dept Anat & Cell Biol Heidelberg Germany D-69120 120 Heidelberg, Germany
Titolo Testata:
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
fascicolo: 3, volume: 33, anno: 2001,
pagine: 461 - 472
SICI:
0022-2828(200103)33:3<461:TNNTIE>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; ANGIOTENSIN-CONVERTING ENZYME; SYMPATHETIC NERVOUS ACTIVITY; HUMAN DOPAMINE TRANSPORTER; FAILING HUMAN HEART; GUINEA-PIG HEART; NEUROPEPTIDE-Y; CALCIUM CHANNELS; UPTAKE SITES; RELEASE;
Keywords:
aortic banding; congestive heart failure; experimental heart failure; norepinephrine transporter; stellate ganglion; sympathetic nerve endings; uptake(1) carrier;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Haass, M Univ Heidelberg, Dept Cardiol, Bergheimer Str 58, D-69115 Heidelberg, Germany Univ Heidelberg Bergheimer Str 58 Heidelberg Germany D-69115 any
Citazione:
J. Backs et al., "The neuronal norepinephrine transporter in experimental heart failure: Evidence for a posttranscriptional downregulation", J MOL CEL C, 33(3), 2001, pp. 461-472

Abstract

An impairment of norepinephrine (NE) re-uptake by the neuronal NE transporter (NET) has been shown to contribute to the increased cardiac net-releaseof NE in congestive heart failure (CHF). The present study investigated which mechanisms are involved in the impairment of NET. Rats with supracoronary aortic banding characterized by myocardial hyper-trophy, elevated left ventricular end diastolic pressures and severe pulmonary congestion were used as an experimental model for CHF. Compared to sham-operated controls, aortic-banded rats had enhanced plasma NE concentrations and decreased cardiacNE stores. In isolated perfused hearts of aortic-banded rats, functional impairment of NET was indicated by a 37% reduction in [B-3]-NE-uptake. In addition, pharmacological blockade of NET with desipramine led to a markedly attenuated increase in the overflow of endogenous NE from hearts of aortic-banded rats. Determination of cardiac NET protein and of NET mRNA in the left stellate ganglion by [H-3]-desipramine binding and competitive RT-PCR, respectively, revealed a 41% reduction of binding sites but no difference ingene expression, The density of sympathetic nerve fibers within the heart was unchanged, as shown by glyoxylic acid-induced histofluorescence. In conclusion, as impairment of intracardiac NE re-uptake by a reduction of NET binding sites is neither mediated by a decreased NET gene expression nor by a loss of noradrenergic nerve terminals, a posttranscriptional downregulation of NET per neuron is suggested in CHF. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 16:28:47