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Titolo:
Voltage-induced activation of mechanosensitive cation channels of leech neurons
Autore:
Menconi, MC; Pellegrini, M; Pellegrino, M;
Indirizzi:
Univ Pisa, Dipartimento Fisiol & Biochim G Moruzzi, I-56127 Pisa, Italy Univ Pisa Pisa Italy I-56127 ol & Biochim G Moruzzi, I-56127 Pisa, Italy Scuola Normale Super Pisa, I-56126 Pisa, Italy Scuola Normale Super Pisa Pisa Italy I-56126 r Pisa, I-56126 Pisa, Italy
Titolo Testata:
JOURNAL OF MEMBRANE BIOLOGY
fascicolo: 1, volume: 180, anno: 2001,
pagine: 65 - 72
SICI:
0022-2631(20010301)180:1<65:VAOMCC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ION CHANNELS; XENOPUS OOCYTES; MEMBRANE; CELLS;
Keywords:
leech; neurons; voltage-sensitivity; stretch-activated channels; growth cone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Pellegrino, M Univ Pisa, Dipartimento Fisiol & Biochim G Moruzzi, Via S Zeno 31, I-56127Pisa, Italy Univ Pisa Via S Zeno 31 Pisa Italy I-56127 56127Pisa, Italy
Citazione:
M.C. Menconi et al., "Voltage-induced activation of mechanosensitive cation channels of leech neurons", J MEMBR BIO, 180(1), 2001, pp. 65-72

Abstract

The voltage dependence of stretch-activated cation channels in leech central neurons was studied in cell-free configurations of the patch-clamp technique. We established that stretch-activated channels excised from identified cell bodies of desheathed ganglia, as well as from neurons in culture, were slowly and reversibly activated by depolarizing membrane potentials. Negative pressure stimuli, applied to the patch pipette during a slow periodical modulation of membrane potential, enhanced channel activity, whereaspositive pressures depressed it. Voltage-induced channel activation was observed, with soft glass pipettes,both in inside-out and outside-out membrane patches, at negative and positive reference potentials, respectively. The results presented in this study demonstrate that membrane depolarization induces slow activation of stretch-activated channels of leech central neurons. This phenomenon is similar to that found in Xenopus oocytes, however, some peculiar features of the voltage dependence in leech stretch-activated channels indicate that specific membrane-glass interactions might not necessarily be involved. Moreover, following depolarization, stretch-activated channels in membrane patches from neurons in culture exhibited significantly shorter delay to activation (sec) than their counterparts from neuronsof freshly isolated ganglia (hundreds of sec).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 15:21:49