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Titolo:
Organization and functional analysis of the mouse transporter associated with antigen processing 2 promoter
Autore:
Arons, E; Kunin, V; Schechter, C; Ehrlich, R;
Indirizzi:
Tel Aviv Univ, George S Wise Fac Life Sci, Dept Cell Res & Immunol, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 mmunol, IL-69978 Tel Aviv, Israel
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 6, volume: 166, anno: 2001,
pagine: 3942 - 3951
SICI:
0022-1767(20010315)166:6<3942:OAFAOT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR HISTOCOMPATIBILITY COMPLEX; MHC-CLASS-I; TUMOR-CELL LINES; HIGHLY ONCOGENIC ADENOVIRUS-12; SEQUENCE BINDING-PROTEIN; IFN-GAMMA; TRANSCRIPTIONAL REGULATION; GENOMIC ORGANIZATION; NEGATIVE SELECTION; ENHANCER SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Ehrlich, R Tel Aviv Univ, George S Wise Fac Life Sci, Dept Cell Res & Immunol, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 69978 Tel Aviv, Israel
Citazione:
E. Arons et al., "Organization and functional analysis of the mouse transporter associated with antigen processing 2 promoter", J IMMUNOL, 166(6), 2001, pp. 3942-3951

Abstract

In accordance with the key role of MHC class I molecules in the adaptive immune response against viruses, they are expressed by most cells, and theirexpression can be enhanced by cytokines. The assembly and cell surface expression of class I complexes depend on a continuous peptide supply. The peptides are generated mainly by the proteasome and are transported to the endoplasmic reticulum by a peptide transport pump consisting of two subunits, TAP1 and TAP2, The proteasome low molecular weight polypeptide (2 and 7), as well as TAP (1 and 2) genes, are coordinately regulated and are induced by IFNs, Despite this coordinate regulation, examination of tumors shows that these genes can be discordantly down-regulated, In pursuing a molecular explanation for these observations, we have characterized the mouse TAP2 promoter region and 5'-flanking sequence, We show that the 5' untranslated regions of TAP2 genes have a characteristic genomic organization that is conserved in both the mouse and the human, The mouse TAP2 promoter belongs to a class of promoters that lack TATA bares hut contain a MED1 (multiple start site element downstream) sequence. Accordingly, transcription is initiated from multiple sites within a 100-nucleotide window, An IFN regulatory factor 1 (IRF1)/IRF2 binding site is located in this region and is involved in both basal and IRF1-induced TAP2 promoter activity, The implication of the extensive differences found among the promoters of class I heavy chain, low molecular weight polypeptide, and TAP genes, all encoding proteins involvedin Ag presentation, is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 07:00:10