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Titolo:
Functional caspase-1 is required for langerhans cell migration and optimalcontact sensitization in mice
Autore:
Antonopoulos, H; Cumberbatch, M; Dearman, RJ; Daniel, RJ; Kimber, I; Groves, RW;
Indirizzi:
Univ Coll London, Dept Med, Ctr Dermatol, London, England Univ Coll London London England Dept Med, Ctr Dermatol, London, England Syngenta Cent Toxicol Lab, Macclesfield, Cheshire, England Syngenta Cent Toxicol Lab Macclesfield Cheshire England heshire, England
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 6, volume: 166, anno: 2001,
pagine: 3672 - 3677
SICI:
0022-1767(20010315)166:6<3672:FCIRFL>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; INTERLEUKIN-1-BETA CONVERTING-ENZYME; DENDRITIC CELLS; FACTOR-ALPHA; HUMAN KERATINOCYTES; ULTRAVIOLET-LIGHT; LYMPH-NODES; TNF-ALPHA; SKIN; IL-1-BETA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Groves, RW Univ Coll London, Middlesex Hosp, Jules Thorn Inst, London Ctr Dermatol, 7th Floor,Mortimer St, London W1T 3AA, England Univ Coll London 7th Floor,Mortimer St London England W1T 3AA
Citazione:
H. Antonopoulos et al., "Functional caspase-1 is required for langerhans cell migration and optimalcontact sensitization in mice", J IMMUNOL, 166(6), 2001, pp. 3672-3677

Abstract

Langerhans cell (LC) migration from epidermis to draining lymph node is a critical first step in cutaneous immune responses. Both TNF-alpha and IL-1 beta are important signals governing this process, but the potential regulatory role of LL-l alpha processing by caspase-1 is unknown, In wild-type (WT) mice, application of the contact allergens 2,4-dinitrofluorobenzine and oxazolone lead to a marked reduction in epidermal LC numbers, but in caspase-1-deficient mice this reduction was not observed. Moreover, although intradermal injection of TNF-alpha (50 ng) induced epidermal LC migration in WTmice, this cytokine failed to induce LC migration in caspase-1-deficient mice. Intradermal IL-1 beta) (50 ng) caused a similar reduction in epidermalLC numbers in both WT and caspase-1-deficient mice, indicating that, givenan appropriate signal, caspase-1-deficient epidermal LC are capable of migration. Contact hypersensitivity to both 2,4-dinitrofluorobenzine and oxazolone was inhibited in caspase-1-deficient mice, indicating a functional consequence of the LC migration defect. In organ culture the caspase-1 inhibitor Ac-YVAD-cmk, but not control peptide, potently inhibited the epidermal LC migration that occurs in this system, and reduced spontaneous migration of LC was observed in skin derived from caspase-1-deficient mice. Moreover, Ac-YVAD-cmk applied to BALB/c mouse skin before application of contact sensitizers inhibited LC migration and contact hypersensitivity in vivo. Taken together, these data indicate that Caspase-1 may play a central role in theregulation of LC migration and suggest that the activity of this enzyme isamenable to control by specific inhibitors both in vivo and in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:24:37