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Titolo:
Cryopreservation of the sperm of the Japanese bitterling
Autore:
Ohta, H; Kawamura, K; Unuma, T; Takegoshi, Y;
Indirizzi:
Natl Res Inst Aquaculture, Nansei, Mie 5160193, Japan Natl Res Inst Aquaculture Nansei Mie Japan 5160193 ei, Mie 5160193, Japan Fukui Prefectural Univ, Fac Biotechnol, Fukui 9170003, Japan Fukui Prefectural Univ Fukui Japan 9170003 technol, Fukui 9170003, Japan
Titolo Testata:
JOURNAL OF FISH BIOLOGY
fascicolo: 3, volume: 58, anno: 2001,
pagine: 670 - 681
SICI:
0022-1112(200103)58:3<670:COTSOT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMMON CARP; SPERMATOZOA; MOTILITY; FISH; OSMOLALITY; POTASSIUM; STORAGE; SALMON; TROUT;
Keywords:
endangered fish; cryoprotectant; cooling rate; cooled temperature;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Ohta, H Natl Res Inst Aquaculture, Nansei, Mie 5160193, Japan Natl Res Inst Aquaculture Nansei Mie Japan 5160193 5160193, Japan
Citazione:
H. Ohta et al., "Cryopreservation of the sperm of the Japanese bitterling", J FISH BIOL, 58(3), 2001, pp. 670-681

Abstract

Sperm of the Japanese bitterling Tanakia limbata that had been cryopreserved with 5 or 10% methanol plus 95 or 90% foetal bovine serum (FBS) showed higher percentage and longer duration of motility than those that had been cryopreserved with 90% FBS and 10% DMSO, glycerol, N,N-dimethylacetamide or N,N-dimethylformamide. Foetal bovine serum, used as extender. had some cryoprotective effects when spermatozoa were cooled either with 10% methanol orwithout methanol. Spermatozoa. cooled to - 40 degreesC and then immersed in liquid nitrogen, had greater post-thaw motility than those cooled to - 20. - 60, or - 80 degreesC. The post-thaw percentage of motile spermatozoa increased significantly (P<0.001) with decreases in the freezing rate from 60to 5<degrees>C min These results indicate that 10% methanol plus 90% foetal bovine serum is a suitable diluent for cryopreservation of bitterling spermatozoa and that samples should be cooled to - 40 degreesC at a low freezing rate for effective storage. (C) 2001 The Fisheries Society of the British Isles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 12:02:34