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Titolo:
Comparative performance of three viral load assays on human immunodeficiency virus type 1 (HIV-1) isolates representing group M (subtypes A to G) andgroup O: LCx HIV RNA Quantitative, AMPLICOR HIV-1 MONITOR version 1.5, andQuantiplex HIV-1 RNA version 3.0
Autore:
Swanson, P; Soriano, V; Devare, SG; Hackett, J;
Indirizzi:
Abbott Labs, AIDS Res & Retrovirus Discovery, Abbott Pk, IL 60064 USA Abbott Labs Abbott Pk IL USA 60064 rus Discovery, Abbott Pk, IL 60064 USA Inst Salud Carlos III, Infect Dis Serv, Madrid, Spain Inst Salud Carlos III Madrid Spain III, Infect Dis Serv, Madrid, Spain
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 3, volume: 39, anno: 2001,
pagine: 862 - 870
SICI:
0095-1137(200103)39:3<862:CPOTVL>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENETIC SUBTYPES; BLOOD-DONORS; PCR ASSAY; PLASMA; QUANTIFICATION; INFECTION; DNA; DIVERSITY; SEQUENCES; STANDARDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Hackett, J Abbott Labs, AIDS Res & Retrovirus Discovery, D-9NG,Bldg AP20,100 Abbott Pk Rd, Abbott Pk, IL 60064 USA Abbott Labs D-9NG,Bldg AP20,100 Abbott Pk Rd Abbott Pk IL USA 60064
Citazione:
P. Swanson et al., "Comparative performance of three viral load assays on human immunodeficiency virus type 1 (HIV-1) isolates representing group M (subtypes A to G) andgroup O: LCx HIV RNA Quantitative, AMPLICOR HIV-1 MONITOR version 1.5, andQuantiplex HIV-1 RNA version 3.0", J CLIN MICR, 39(3), 2001, pp. 862-870

Abstract

The performance of the LCx HIV RNA Quantitative (LCx HIV), AMPLICOR HIV-1 MONITOR version 1.5 (MONITOR v1.5), and Quantiplex HIV-1 RNA version 3.0 (bDNA v3.0) viral load assays aas evaluated with 39 viral isolates (3 A, 7 B,6 C, 4 D, 8 E, 4 F, 1 G, 4 mosaic, and 2 group O). Quantitation across theassay dynamic ranges was assessed using serial fivefold dilutions of the viruses. In addition, sequences of gag-encoded p24 (gag p24),pol-encoded integrase, and env-encoded gp41 were analyzed to assign group and subtype and to assess nucleotide mismatches at primer and probe binding sites. For group M isolates, quantification was highly correlated among all three assays. In contrast, only the LCx HN assay reliably quantified group O isolates. The bDNA v3.0 assay detected hut consistently underquantified group O viruses, whereas the MONITOR v1.5 test failed to detect group O viruses. Analysis of target regions revealed fewer primer or probe mismatches in the LCx HIV assay than in the MONITOR v1.5 test. Consistent with the high level of nucleotide conservation is the ability of the LCx HIV assay to quantify efficiently human immunodeficiency virus type 1 group M and the genetically diverse group O.

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Documento generato il 01/04/20 alle ore 11:30:39