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Titolo:
Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system
Autore:
Townsend, KM; Boyce, JD; Chung, JY; Frost, AJ; Adler, B;
Indirizzi:
Univ Queensland, Sch Vet Sci, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 Brisbane, Qld 4072, Australia Monash Univ, Dept Microbiol, Bacterial Pathogenesis Res Grp, Clayton, Vic 3800, Australia Monash Univ Clayton Vic Australia 3800 Grp, Clayton, Vic 3800, Australia
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 3, volume: 39, anno: 2001,
pagine: 924 - 929
SICI:
0095-1137(200103)39:3<924:GOOPMC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIOSYNTHETIC LOCUS; MOLECULAR-CLONING; IDENTIFICATION; SYNTHASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Townsend, KM Univ Queensland, Sch Vet Sci, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 4072, Australia
Citazione:
K.M. Townsend et al., "Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system", J CLIN MICR, 39(3), 2001, pp. 924-929

Abstract

Current serotyping methods classify Pasteurella multocida into five capsular serogroups (serogroups A, B, D, E, and F) and 16 somatic serotypes (serotypes 1 to 16), In the present study, we have developed a multiplex PCR assay as a rapid alternative to the conventional capsular serotyping system. The serogroup-specific primers used in this assay were designed following identification, sequence determination, and analysis of the capsular biosynthetic loci of each capsular serogroup, The entire capsular biosynthetic lociof P. multocicia A:1 (X-73) and B:2 (M1401) have been cloned and sequencedpreviously (J, Y. Chung, Y, M. Zhang, and B. Adler, FEMS Microbiol. Lett, 166:289-296, 1998; J. D. Boyce, J, Y. Chung, and B, Adler, Vet. Microbiol, 72:121-134, 2000), Nucleotide sequence analysis of the biosynthetic region (region 2) from each of the remaining three serogroups, serogroups D, E, and F, identified serogroup-specific regions and gave an indication of the capsular polysaccharide composition. The multiplex capsular PCR assay was highly specific, and its results, with the exception of those for some serogroup P strains, correlated well with conventional serotyping results, Sequence analysis of the strains that gave conflicting results confirmed the validity of the multiplex PCR and indicated that these strains were in fact capsular serogroup A. The multiplex PCR will clarify the distinction between closely related serogroups A and F and constitutes a rapid assay for the definitive classification of P. multocida capsular types.Current serotyping methods classify Pasteurella multocida into five capsular serogroups (serogroups A, B, D, E, and Fl and 16 somatic serotypes (serotypes I to 16), In the present study, we have developed a multiplex PCR assay as a rapid alternative to the conventional capsular serotyping system. The serogroup-specific primers used in this assay were designed following identification, sequence determination, and analysis of the capsular biosynthetic loci of each capsular serogroup, The entire capsular biosynthetic loci of P. multocida A:1 (X-73) and B:2 (M1404) have been cloned and sequenced previously (J, Y. Chung, Y, M. Zhang, and B. Adler, FEMS Microbiol. Lett, 166:289-296, 1998; J. D. Boyce, J, Y. Chung, and B, Adler, Vet. Microbiol, 72:121-134, 2000), Nucleotide sequence analysis of the biosynthetic region (region 2) from each of the remaining three serogroups, serogroups D, E, and F, identified serogroup-specific regions and gave an indication of the capsular polysaccharide composition. The multiplex capsular PCR assay was highly specific, and its results, with the exception of those for some serogroup P strains, correlatedwell with conventional serotyping results, Sequence analysis of the strains that gave conflicting results confirmed the validity of the multiplex PCRand indicated that these strains were in fact capsular serogroup A. The multiplex PCR will clarify the distinction between closely related serogroupsA and F and constitutes a rapid assay for the definitive classification ofP. multocida capsular types.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 18:28:14