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Titolo:
Evidence of productively infected CD8(+) T cells in patients with AIDS: Implications for HIV-1 pathogenesis
Autore:
Saha, K; Zhang, JC; Zerhouni, B;
Indirizzi:
Ohio State Univ, Childrens Res Inst, Dept Pediat & Mol Virol Immunol & MedGenet, Med Ctr, Columbus, OH 43205 USA Ohio State Univ Columbus OH USA 43205 et, Med Ctr, Columbus, OH 43205 USA
Titolo Testata:
JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
fascicolo: 3, volume: 26, anno: 2001,
pagine: 199 - 207
SICI:
1525-4135(20010301)26:3<199:EOPICT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HERPESVIRUS-SAIMIRI; SOLUBLE FACTOR(S); BETA-CHEMOKINES; TYPE-1 HIV-1; LYMPHOCYTES; CLONES; CD4(+); IMMORTALIZATION; REPLICATION;
Keywords:
CD8(+); lymphocytes; HIV-1 tropism; syncitium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Saha, K Ohio State Univ, Childrens Res Inst, Dept Pediat & Mol Virol Immunol & MedGenet, Med Ctr, 700 Childrens Dr,Room W532, Columbus, OH 43205 USA Ohio State Univ 700 Childrens Dr,Room W532 Columbus OH USA 43205 A
Citazione:
K. Saha et al., "Evidence of productively infected CD8(+) T cells in patients with AIDS: Implications for HIV-1 pathogenesis", J ACQ IMM D, 26(3), 2001, pp. 199-207

Abstract

CD8(+) T lymphocytes play an important protective role against HIV infection. The onset of AIDS is associated with a decline in both the number of CD8(+) T lymphocytes and anti-HIV cytotoxic activity in CD8(+) T cells. The reason for this progressive failure of CD8+ T cells in HIV-1 infection remains unknown. Earlier reports have shown presence of viral DNA in CD8(+) cells of HIV-1-infected patients: under some conditions, CD8(+) T cells have been shown to express CD4 in vitro and can be susceptible to infection with HIV-1. However, whether CD8(+) lymphocytes in vivo can be productively infected with HIV-1 remains unclear. In this study, we generated multiple CD8(+)T-cell clones from two patients with AIDS. These clones were CD8(+)/CD3(+)but did not express CD4. Several of these CD8(+) clones from both patientswere found to be endogenously infected with HIV-1 and spontaneously produced these viruses. CD8(+) cell-produced HIV-1 was biologically competent because viruses produced by most of these clones could efficiently infect and replicate in peripheral blood lymphocytes from HIV-negative donors. In addition, some of these viruses were able to form syncytia in MT-2 cells indicating syncytium-inducing phenotype. Comparison of the sequences in V3 loop areas among different viruses showed changes in some of the clones from bothpatients. For the first time, this report provides direct evidence that mature CD8(+) T cells can be productively infected with HIV-1 in patients with AIDS. Direct infection of CD8(+) T lymphocytes may play a role in the eventual failure of these cells in HIV-1 infection.

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Documento generato il 04/12/20 alle ore 12:41:30