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Titolo:
Relationships between clonogenic cell survival, DNA damage and chromosomalradiosensitivity in nine human cervix carcinoma cell lines
Autore:
Eastham, AM; Atkinson, J; West, CML;
Indirizzi:
Christie Hosp NHS Trust, Paterson Inst Canc Res, CRC Expt Radiat Oncol Grp, Manchester M20 4BX, Lancs, England Christie Hosp NHS Trust Manchester Lancs England M20 4BX , Lancs, England
Titolo Testata:
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
fascicolo: 3, volume: 77, anno: 2001,
pagine: 295 - 302
SICI:
0955-3002(200103)77:3<295:RBCCSD>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRAND BREAKS; HUMAN-TUMOR-CELLS; MICRONUCLEUS ASSAY; INTRINSIC RADIOSENSITIVITY; GEL-ELECTROPHORESIS; PRIMARY CULTURES; REPAIR FIDELITY; INDUCTION; RADIOTHERAPY; FIBROBLASTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: West, CML Christie Hosp NHS Trust, Paterson Inst Canc Res, CRC Expt RadiatOncol Grp, Wilmslow Rd, Manchester M20 4BX, Lancs, England Christie Hosp NHS Trust Wilmslow Rd Manchester Lancs England M20 4BX
Citazione:
A.M. Eastham et al., "Relationships between clonogenic cell survival, DNA damage and chromosomalradiosensitivity in nine human cervix carcinoma cell lines", INT J RAD B, 77(3), 2001, pp. 295-302

Abstract

Purpose: To compare clonogenic cell survival, DNA damage and chromosomal radiosensitivity in nine cervix carcinoma cell lines. Materials and methods: Initial and residual (after 24 h repair) radiation-induced DNA damage was evaluated using pulsed field gel electrophoresis. Chromosome damage was measured by micronucleus (MN) induction in cytochalasin-B-induced binucleate cells. Results: Significant differences between the cell lines were obtained in the induced levels of initial damage, residual damage and MN. Values for SF2for the nine cell lines ranged from 0.36 to 0.92. No correlation was foundbetween clonogenic measurements of radiosensitivity and initial DNA damagedose-response slopes. However, borderline significant correlations were seen between clonogenic radiosensitivity data and the levels of residual DNA damage. There was no correlation between clonogenic radiosensitivity and the levels of radiation-induced MN. Cell lines with high levels of initial damage had high yields of MN induced by radiation and the correlation seen was significant. Conclusions: The poor correlation between the different endpoints precludes their use in a clinical setting on primary tumour samples in vitro. It may be that tumour cell lines in vitro are a poor model for rumours in vivo. Studies aimed at assessing assays for measuring tumour radiosensitivity therefore should employ clinical samples. In vitro cell line work should concentrate on unravelling the complex mechanisms involved in determining a radiosensitive or radioresistant phenotype.

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Documento generato il 05/07/20 alle ore 03:52:51