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Titolo:
Monitoring of ascorbate at a constant rate in cell culture: Effect on cellgrowth
Autore:
Chepda, T; Cadau, M; Girin, P; Frey, J; Chamson, A;
Indirizzi:
Fac Med, Biochim Lab, F-42023 St Etienne 2, France Fac Med St Etienne France 2 d, Biochim Lab, F-42023 St Etienne 2, France
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 1, volume: 37, anno: 2001,
pagine: 26 - 30
SICI:
1071-2690(200101)37:1<26:MOAAAC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-SKIN FIBROBLASTS; HUMAN DERMAL FIBROBLASTS; COLLAGEN-SYNTHESIS; CATALYTIC METALS; ACID 2-PHOSPHATE; FREE-RADICALS; VITAMIN-C; PROLIFERATION; METABOLISM;
Keywords:
ascorbate; ascorbate-phosphate; constant rate; cell growth;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Chamson, A Fac Med, Biochim Lab, 15 Rue Ambroise Pare, F-42023 St Etienne 2, France Fac Med 15 Rue Ambroise Pare St Etienne France 2 nne 2, France
Citazione:
T. Chepda et al., "Monitoring of ascorbate at a constant rate in cell culture: Effect on cellgrowth", IN VITRO-AN, 37(1), 2001, pp. 26-30

Abstract

Ascorbic acid (vitamin C) is a primary antioxidant for cells. But, ascorbic acid added to culture medium is not readily available to cells in culture, because it is unstable in aqueous media. We determined the conditions required to obtain and maintain a constant concentration of ascorbate in the culture medium using ascorbate and ascorbate-phosphate. The study was carried out with human fibroblasts and the amounts of ascorbate in the culture medium were determined by high performance liquid chromatography. A mixture of 0.25 mmol/L ascorbate and 0.45 mmol/L ascorbate-phosphate provided a constant concentration of ascorbate in the culture medium. This constant ascorbate concentration proved to be nontoxic for cells and stimulated cell growth in the short term and long term.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 06:01:54