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Titolo:
Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes
Autore:
Sif, S; Saurin, AJ; Imbalzano, AN; Kingston, RE;
Indirizzi:
Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA Massachusetts Gen Hosp Boston MA USA 02114 Mol Biol, Boston, MA 02114 USA Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 ch Med, Dept Genet, Boston, MA 02115 USA Ohio State Univ, Coll Med, Dept Mol & Cellular Biochem, Columbus, OH 43210USA Ohio State Univ Columbus OH USA 43210 ular Biochem, Columbus, OH 43210USA Univ Massachusetts, Sch Med, Dept Cell Biol, Worcester, MA 01655 USA Univ Massachusetts Worcester MA USA 01655 l Biol, Worcester, MA 01655 USA
Titolo Testata:
GENES & DEVELOPMENT
fascicolo: 5, volume: 15, anno: 2001,
pagine: 603 - 618
SICI:
0890-9369(20010301)15:5<603:PACOMB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
YEAST SWI/SNF COMPLEX; SWI-SNF COMPLEX; HISTONE DEACETYLASE; CELL-CYCLE; TRANSCRIPTIONAL REPRESSION; GLUCOCORTICOID RECEPTOR; RETINOBLASTOMA PROTEIN; NUCLEOSOME DISRUPTION; ACTIVATOR BINDING; DNA;
Keywords:
chromatin remodeling; ATPase activity; histone deacetylases; Sin3 repressor complex; human SWI/SNF complexes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Sif, S Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA Massachusetts Gen Hosp Boston MA USA 02114 l, Boston, MA 02114 USA
Citazione:
S. Sif et al., "Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes", GENE DEV, 15(5), 2001, pp. 603-618

Abstract

Alteration of nucleosomes by ATP-dependent remodeling complexes representsa critical step in the regulation of transcription. The human SWI/SNF (hSWI/SNF) family is composed of complexes that contain either Brg1 or hBrm as the central ATPase; however, these separate complexes have not been compared functionally. Here we describe the establishment of cell lines that express epitolpe-tagged Brg1 and hBrm and a characterization of the complexes associated with these two ATPases. We show that Brg1 fractionates into two complexes that differ in activity and subunit composition, whereas hBrm is found in one complex with lower activity than the Brg1 complexes. These threecomplexes can remodel nucleosomal arrays, increase restriction enzyme accessibility, and hydrolyze ATP in a DNA-dependent manner. The three complexesdiffer markedly in their ability to remodel mononucleosomal core particles. We also show that the hBrm complex and one of the Brg1 complexes contain components of the mammalian Sin3 (mSin3) complex. In addition, we have found that Brg1, hBrm, and BAF155 can interact specifically with mSin3A in vitro, showing a direct association of hSWI/SNF complexes with proteins involved in gene repression. These unexpected functional characteristics indicate that these hSWI/SNF complexes play diverse regulatory roles.

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Documento generato il 25/01/20 alle ore 18:40:11