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Titolo:
C-elegans mre-11 is required for meiotic recombination and DNA repair but is dispensable for the meiotic G(2) DNA damage checkpoint
Autore:
Chin, GM; Villeneuve, AM;
Indirizzi:
Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 Dept Dev Biol, Stanford, CA 94305 USA Stanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 d, Dept Genet, Stanford, CA 94305 USA
Titolo Testata:
GENES & DEVELOPMENT
fascicolo: 5, volume: 15, anno: 2001,
pagine: 522 - 534
SICI:
0890-9369(20010301)15:5<522:CMIRFM>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRAND-BREAK; SACCHAROMYCES-CEREVISIAE; CAENORHABDITIS-ELEGANS; ATAXIA-TELANGIECTASIA; CROSSING-OVER; CELL-CYCLE; GENETIC REQUIREMENTS; TELOMERE MAINTENANCE; CHROMOSOME SYNAPSIS; VERTEBRATE CELLS;
Keywords:
meiosis; recombination; DNA repair; checkpoint; C. elegans; Mre11; mre-11;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Villeneuve, AM Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 Stanford, CA 94305 USA
Citazione:
G.M. Chin e A.M. Villeneuve, "C-elegans mre-11 is required for meiotic recombination and DNA repair but is dispensable for the meiotic G(2) DNA damage checkpoint", GENE DEV, 15(5), 2001, pp. 522-534

Abstract

We investigated the roles of Caenorhabditis elegans MRE-11 in multiple cellular processes required to maintain genome integrity. Although yeast Mre11is known to promote genome stability through several diverse pathways, inviability of vertebrate cells that lack Mre11 has hindered elucidation of the in vivo roles of this conserved protein in metazoan biology. Worms homozygous for an mre-11 null mutation are viable, allowing us to demonstrate in vivo requirements for MRE-11 in meiotic recombination and DNA repair. In mre-11 mutants, meiotic crossovers are not detected, and oocyte chromosomes lack chiasmata but appear otherwise intact, gamma -irradiation of mre-11 mutant germ cells during meiotic prophase eliminates progeny survivorship and induces chromosome fragmentation and other cytologically visible abnormalities, indicating a defect in repair of radiation-induced chromosome damage. Whereas mre-11 mutant germ cells are repair-deficient, they retain functionof the meiotic G(2) DNA damage checkpoint that triggers germ cell apoptosis in response to ionizing radiation. Although mre-11/mre-11 animals derivedfrom heterozygous parents are viable and produce many embryos, there is a marked drop both in the number and survivorship of embryos produced by succeeding generations. This progressive loss of fecundity and viability indicates that MRE-11 performs a function essential for maintaining reproductive capacity in the species.

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Documento generato il 23/09/20 alle ore 09:11:08