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Titolo:
Augmentation of myocardial transfection using TerplexDNA: a novel gene delivery system
Autore:
Affleck, DG; Yu, L; Bull, DA; Bailey, SH; Kim, SW;
Indirizzi:
Ctr Controlled Chem Delivery, Dept Pharmaceut & Pharmaceut Chem, Salt LakeCity, UT 84132 USA Ctr Controlled Chem Delivery Salt Lake City UT USA 84132 ty, UT 84132 USA Univ Utah, Hlth Sci Ctr, Dept Surg, Div Cardiothorac Surg, Salt Lake City,UT USA Univ Utah Salt Lake City UT USA Cardiothorac Surg, Salt Lake City,UT USA
Titolo Testata:
GENE THERAPY
fascicolo: 5, volume: 8, anno: 2001,
pagine: 349 - 353
SICI:
0969-7128(200103)8:5<349:AOMTUT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; VIRAL VECTORS; THERAPY; RECEPTOR; CELLS;
Keywords:
transfection; gene delivery; myocardium; TerplexDNA; direct injection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, SW Ctr Controlled Chem Delivery, Dept Pharmaceut & Pharmaceut Chem, BPRB RM 205, Salt Lake City, UT 84132 USA Ctr Controlled Chem Delivery BPRB RM 205 Salt Lake City UT USA 84132
Citazione:
D.G. Affleck et al., "Augmentation of myocardial transfection using TerplexDNA: a novel gene delivery system", GENE THER, 8(5), 2001, pp. 349-353

Abstract

Gene therapy is a potential new strategy for the treatment of cardiovascular disease. The most efficacious method of gene delivery remains a key hurdle to effective gene therapy. We present the application of a novel, nonviral gene delivery system (TerplexDNA) to augment myocardial transfection. The hearts of New Zealand white rabbits were injected with reporter genes, luciferase cDNA or beta -galactosidase cDNA, either as naked plasmid DNA or plasmid DNA complexed with stearyl-poly(L-lysine)-low density lipoprotein (TerplexDNA). Three day left heart myocardial cell lysates produced 44571 +/-8730 RLU (RLU = total light units/mg protein) for the TerplexDNA luciferase rabbits versus 1638 +/- 567 RLU for the naked luciferase rabbits (P = 0.002). Thirty days after injection, myocardial lysates produced 677 +/- 52 RLU for the TerplexDNA luciferase hearts versus 18 +/- 3 RLU for the naked luciferase hearts (P = 0.002). Histologic analysis of the hearts transfected with beta -galactosidade showed that TerplexDNA increased the area and depth of transfection compared with the naked plasmid DNA alone. The hearts of Sprague-Dawley rats were injected in a similar fashion and analyzed at 1, 3, 5 10, 15, 25 and 30 days after injection. The naked luciferase injected hearts showed transient elevation of luciferase activity to day 5 but fell back to baseline levels after that time-point. The TerplexDNA luciferase injected hearts had significantly elevated luciferase activity to 30 days. TheTerplex gene delivery system significantly augments myocardial transfection compared with a naked plasmid DNA system alone. The advantage in transfection efficiency appears to be related to the unique properties of the TerplexDNA carrier molecule. The TerplexDNA delivery system represents a novel means to augment transfection of the myocardium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 05:38:03