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Titolo:
The Streptomyces venezuelae pikAV gene contains a transcription unit essential for expression of enzymes involved in glycosylation of narbonolide and10-deoxymethynolide
Autore:
Chen, S; Roberts, JB; Xue, YQ; Sherman, DH; Reynolds, KA;
Indirizzi:
Virginia Commonwealth Univ, Dept Med Chem, Richmond, VA 23219 USA VirginiaCommonwealth Univ Richmond VA USA 23219 , Richmond, VA 23219 USA Virginia Commonwealth Univ, Inst Struct Biol & Drug Discovery, Richmond, VA 23219 USA Virginia Commonwealth Univ Richmond VA USA 23219 , Richmond, VA 23219 USA Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 robiol, Minneapolis, MN 55455 USA Univ Minnesota, Biol Proc Technol Inst, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 l Inst, Minneapolis, MN 55455 USA
Titolo Testata:
GENE
fascicolo: 1-2, volume: 263, anno: 2001,
pagine: 255 - 264
SICI:
0378-1119(20010124)263:1-2<255:TSVPGC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; BACILLUS-SUBTILIS; BIOSYNTHETIC GENE; MESSENGER-RNA; ERYTHROMYCIN; TERMINATION; REDUCTASE; MACROLIDE; CLUSTER;
Keywords:
thioesterases; polyketide; glycosylation; pikromycin; Venezuelae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Reynolds, KA Virginia Commonwealth Univ, Dept Med Chem, 800 E Leigh St, Richmond, VA 23219 USA Virginia Commonwealth Univ 800 E Leigh St Richmond VA USA 23219
Citazione:
S. Chen et al., "The Streptomyces venezuelae pikAV gene contains a transcription unit essential for expression of enzymes involved in glycosylation of narbonolide and10-deoxymethynolide", GENE, 263(1-2), 2001, pp. 255-264

Abstract

In Streptomyces venezuelae, four polyketide synthase (PKS) polypeptides encoded by pikAI-pikAIV are used to generate 10 and 12-membered macrocyclic structures, narbonolide and 10-deoxymethynolide. Sequence analysis suggests these genes are translationally coupled with downstream genes, pikAV (encoding a type II thioesterase), desVIII-desVI (encoding enzymes responsible for production of the final glycosylated products pikromycin, narbomycin, methymycin and neomethymycin) and desR (a resistance gene). Type II thioesterases have been suggested to have an editing function in polyketide biosynthesis and deletion of the corresponding genes often leads to decreased levelsof polyketide production. Surprisingly an in-frame deletion of 687 bp of the 843 bp pikAV ORF led to a strain SC1022 that produced normal yields of polyketide products, but only in the aglycone form. Plasmid-based expressionof the desVIII-VI and desR in the SC1022 strain completely restored production of glycosylated products, despite the absence of a functional pikAV gene product. Under these conditions the PikAV TEII therefore does not play an important role in polyketide biosynthesis, and its function remains an enigma. These observations also demonstrate that the region of pikAV DNA deleted in strain SC1022 contains a transcription unit essential for expressionof the des genes. A sequence alignment of PikAV with members of the highlyconserved type II thioesterases revealed a short divergent region at the carboxy terminus, suggesting a region of pikAV that might contain such a transcription unit. DNA containing this region of pikAV was shown to be able to increase plasmid-based expression of both crotonyl CoA reductase gene (ccr) and the erythromycin resistance gene (ermE) in S. venezuelae. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:03:01