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Titolo:
Discovery of new human beta-defensins using a genomics-based approach
Autore:
Jia, HP; Schutte, BC; Schudy, A; Linzmeier, R; Guthmiller, JM; Johnson, GK; Tack, BF; Mitros, JP; Rosenthal, A; Ganz, T; McCray, PB;
Indirizzi:
Univ Iowa Hosp & Clin, Dept Pediat, Iowa City, IA 52242 USA Univ Iowa Hosp& Clin Iowa City IA USA 52242 iat, Iowa City, IA 52242 USA Univ Iowa Hosp & Clin, Genet PhD Program, Iowa City, IA 52242 USA Univ Iowa Hosp & Clin Iowa City IA USA 52242 ram, Iowa City, IA 52242 USA Univ Iowa Hosp & Clin, Dept Microbiol, Iowa City, IA 52242 USA Univ Iowa Hosp & Clin Iowa City IA USA 52242 iol, Iowa City, IA 52242 USA Univ Iowa, Coll Dent, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Iowa, Coll Dent, Iowa City, IA 52242 USA Genome Sequencing Ctr, Inst Mol Biotechnol, Jena, Germany Genome Sequencing Ctr Jena Germany , Inst Mol Biotechnol, Jena, Germany Univ Calif Los Angeles, Sch Med, Dept Med, Los Angeles, CA USA Univ Calif Los Angeles Los Angeles CA USA Dept Med, Los Angeles, CA USA Univ Calif Los Angeles, Sch Med, Dept Pathol, Los Angeles, CA USA Univ Calif Los Angeles Los Angeles CA USA pt Pathol, Los Angeles, CA USA Univ Calif Los Angeles, Sch Med, Will Rogers Inst Pulm Res, Los Angeles, CA USA Univ Calif Los Angeles Los Angeles CA USA Pulm Res, Los Angeles, CA USA
Titolo Testata:
GENE
fascicolo: 1-2, volume: 263, anno: 2001,
pagine: 211 - 218
SICI:
0378-1119(20010124)263:1-2<211:DONHBU>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIMICROBIAL PEPTIDES; HUMAN BETA-DEFENSIN-1; HUMAN EPIDIDYMIS; GENE-EXPRESSION; EPITHELIA; SECRETION; CLONING; PLASMA; TISSUE; LUNG;
Keywords:
bacterial artificial chromosome; interleukin-1 beta; NF kappa B; contiguous sequence; chromosome 8;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: McCray, PB Univ Iowa Hosp & Clin, Dept Pediat, 200 Hawkins Dr, Iowa City, IA 52242 USA Univ Iowa Hosp & Clin 200 Hawkins Dr Iowa City IA USA 52242 USA
Citazione:
H.P. Jia et al., "Discovery of new human beta-defensins using a genomics-based approach", GENE, 263(1-2), 2001, pp. 211-218

Abstract

Epithelial beta -defensins are broad-spectrum cationic antimicrobial peptides that also act as chemokines for adaptive immune cells. In the human genome, all known defensin genes cluster to a <1 Mb region of chromosome 8p22-p23. To identify new defensin genes, the DNA sequence from a contig of large-insert genomic clones from the region containing human <beta>-defensin-2 (HBD-2) was analyzed for the presence of defensin genes. This sequence survey identified a novel beta -defensin, termed HBD-3. The HBD-3 gene containstwo exons, is located 13 kb upstream from the HBD-2 gene, and it is transcribed in the same direction. A partial HBD-3 cDNA clone was amplified from cDNA derived from IL-1 beta induced fetal lung tissue. The cDNA sequence encodes for a 67 amino acid peptide that is similar to 43% identical to HBD-2and shares the beta -defensin six cysteine motif. By PCR analysis of two commercial cDNA panels, HBD-3 expression was detected in adult heart, skeletal muscle, placenta and in fetal thymus. From RT-PCR experiments, HBD-3 expression was observed in skin, esophagus, gingival keratinocytes, placenta and trachea. Furthermore, in fetal lung explants and gingival keratinocytes,HBD-3 mRNA expression was induced by IL-1 beta. Additional sequence analysis identified the HE2 (human epididymis secretory protein) gene 17 kb upstream from the HBD-3 gene. One splice variant of this gene (HE2 beta1) encodes a beta -defensin consensus cysteine motif, suggesting it represents a defensin gene product. HE2 beta1 mRNA expression was detected in gingival keratinocytes and bronchial epithelia using RT-PCR analysis. The discovery of these novel beta -defensin genes may allow further understanding of the roleof defensins in host immunity at mucosal surfaces. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:09:31