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Titolo:
16S rDNA diversity of cultured and uncultured prokaryotes of a mat sample from Lake Fryxell, McMurdo Dry Valleys, Antarctica
Autore:
Brambilla, E; Hippe, H; Hagelstein, A; Tindall, BJ; Stackebrandt, E;
Indirizzi:
DSMZ Deutsch Sammlung Mikroorganismen & Zellkultu, D-38124 Braunschweig, Germany DSMZ Deutsch Sammlung Mikroorganismen & Zellkultu Braunschweig Germany D-38124
Titolo Testata:
EXTREMOPHILES
fascicolo: 1, volume: 5, anno: 2001,
pagine: 23 - 33
SICI:
1431-0651(200102)5:1<23:1RDOCA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOUTHERN VICTORIA LAND; RIBOSOMAL-RNA GENE; SP-NOV; SEA-ICE; PSYCHROPHILIC BACTERIA; MEROMICTIC LAKES; MARINE-SALINITY; TAYLOR VALLEY; COMB. NOV; ACE LAKE;
Keywords:
biodiversity; Lake Fryxell mat; Antarctica; psychrophiles;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Stackebrandt, E DSMZ Deutsch Sammlung Mikroorganismen & Zellkultu, Mascheroder Weg 1B, D-38124 Braunschweig, Germany DSMZ Deutsch Sammlung Mikroorganismen & Zellkultu Mascheroder Weg 1B Braunschweig Germany D-38124
Citazione:
E. Brambilla et al., "16S rDNA diversity of cultured and uncultured prokaryotes of a mat sample from Lake Fryxell, McMurdo Dry Valleys, Antarctica", EXTREMOPHIL, 5(1), 2001, pp. 23-33

Abstract

The prokaryotic diversity of aerobic and anaerobic bacterial isolates and of bacterial and archaeal 16S rDNA clones was determined for a microbial mat sample from the moated region of Lake Fryxell, McMurdo Dry Valleys, Antarctica. Among the anaerobic bacteria, members of Clostridium estertheticum and some other psychrotolerant strains dominated whereas methanogens and other Archaea were lacking. Isolates highly related to Flavobacterium hibernum, Janthinobacterium lividum, and Arthrobacter flavus were among the aerobicbacteria most frequently isolated. Assessment of more than 350 partial 16SrDNA clone sequences of libraries generated by Bacteria- and Archaea-specific PCR primers revealed a rich spectrum of bacterial diversity but only two different archaeal clone sequences. Among the Bacteria, representative sequences belonged to the class Proteobacteria, order Verucomicrobiales, class Actinobacteria, Clostridium/Bacillus subphylum of Gram-positives, and theCytophaga-Flavobacterium-Bacteroides phylum. The clones formed about 70 higher taxonomy groups (<98% sequence similarity) and 133 potential species, i.e., groups of clones sharing greater than 98% similarity. Only rarely were clone sequences found to be highly related to Lake Fryxell isolates and to strains of described species. Subsequent analysis of ten sequencing batches of 36 individual clones indicated that the diversity might be still higher than had been assessed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 07:34:33