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Titolo:
Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase inresponse to osmotic stress
Autore:
Proft, M; Pascual-Ahuir, A; de Nadal, E; Arino, J; Serrano, R; Posas, F;
Indirizzi:
UPF, Dept Ciencias Expt Salut, Cell Signaling Unit, E-08003 Barcelona, Spain UPF Barcelona Spain E-08003 ell Signaling Unit, E-08003 Barcelona, Spain Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022, Spain Univ Politecn Valencia Valencia Spain 46022 antas, Valencia 46022, Spain Univ Autonoma Barcelona, Fac Vet, Dept Bioquim Biol Mol, E-08193 Barcelona, Spain Univ Autonoma Barcelona Barcelona Spain E-08193 E-08193 Barcelona, Spain
Titolo Testata:
EMBO JOURNAL
fascicolo: 5, volume: 20, anno: 2001,
pagine: 1123 - 1133
SICI:
0261-4189(20010301)20:5<1123:ROTSTR>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; YEAST SACCHAROMYCES-CEREVISIAE; NUCLEAR EXPORT; BZIP PROTEIN; PATHWAYS; GENE; INDUCTION; MSN2P; OSMOSENSOR; BINDING;
Keywords:
Hog1; mitogen-activated protein kinase; signal transduction; Sko1; stress response;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Posas, F UPF, Dept Ciencias Expt Salut, Cell Signaling Unit, E-08003 Barcelona, Spain UPF Barcelona Spain E-08003 ling Unit, E-08003 Barcelona, Spain
Citazione:
M. Proft et al., "Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase inresponse to osmotic stress", EMBO J, 20(5), 2001, pp. 1123-1133

Abstract

Exposure of yeast to increases in extracellular osmolarity activates the Hog1 mitogen-activated protein kinase (MAPK), which is essential for the induction of gene expression required for cell survival upon osmotic stress. Several genes are regulated in response to osmotic stress by Sko1, a transcriptional repressor of the ATF/CREB family. We show by in vivo coprecipitation and phosphorylation studies that Sko1 and Hog1 interact and that Shot isphosphorylated upon osmotic stress in a Hog1-dependent manner. Hog1 phosphorylates Sko1 in vitro at multiple sites within the N-terminal region. Phosphorylation of Sko1 disrupts the Sko1-Ssn6-Tup1 repressor complex, and consistently, a mutant allele of Sko1, unphosphorylatable by Hog1, exhibits less derepression than the wild type. Interestingly, Sko1 repressor activity is further enhanced in strains with high protein kinase A (PKA) activity. PKA phosphorylates Sko1 near the bZIP domain and mutation of these sites eliminates modulation of Sko1 responses to high PKA activity. Thus, Sko1 transcriptional repression is controlled directly by the Hog1 MAPK in response tostress, and this effect is further modulated by an independent signaling mechanism through the PKA pathway.

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Documento generato il 14/07/20 alle ore 09:31:52