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Titolo:
A single amino acid change within antigenic domain II of the spike proteinof bovine coronavirus confers resistance to virus neutralization
Autore:
Yoo, DW; Deregt, D;
Indirizzi:
Univ Guelph, Ontario Vet Coll, Dept Pathobiol, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 athobiol, Guelph, ON N1G 2W1, Canada Agr Canada, Anim Dis Res Inst, Lethbridge, AB T1J 3Z4, Canada Agr Canada Lethbridge AB Canada T1J 3Z4 t, Lethbridge, AB T1J 3Z4, Canada
Titolo Testata:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
fascicolo: 2, volume: 8, anno: 2001,
pagine: 297 - 302
SICI:
1071-412X(200103)8:2<297:ASAACW>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS; MONOCLONAL-ANTIBODIES; MURINE CORONAVIRUS; SEQUENCE-ANALYSIS; ENTERIC CORONAVIRUSES; PROTEOLYTIC CLEAVAGE; STRUCTURAL PROTEINS; TRYPSIN TREATMENT; WINTER DYSENTERY; RECEPTOR-BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Yoo, DW Univ Guelph, Ontario Vet Coll, Dept Pathobiol, Guelph, ON N1G 2W1,Canada Univ Guelph Guelph ON Canada N1G 2W1 , Guelph, ON N1G 2W1, Canada
Citazione:
D.W. Yoo e D. Deregt, "A single amino acid change within antigenic domain II of the spike proteinof bovine coronavirus confers resistance to virus neutralization", CL DIAG LAB, 8(2), 2001, pp. 297-302

Abstract

The spike glycoprotein is a major neutralizing antigen of bovine coronavirus (BCV). Conformational neutralizing epitopes of group A and group B monoclonal antibodies (MAbs) have previously been mapped to two domains at aminoacids 351 to 403 (domain I) and amino acids 517 to 621 (domain II). To further map antigenic sites, neutralization escape mutants of BCV were selected with a group A MAb which has both in vitro and in vivo virus-neutralizingability. The escape mutants were demonstrated to be neutralization resistant to the selecting group A MAb and remained sensitive to neutralization bya group B MAb. In radioimmunoprecipitation assays, the spike proteins of neutralization escape mutants were shown to have lost their reactivities with the selecting group A MAb. Sequence analysis of the spike protein genes of the escape mutants identified a single nucleotide substitution of C to T at position 1583, resulting in the change of alanine to valine at amino acid position 528 (A528V). The mutation occurs in domain II and in a location which corresponds to the hypervariable region of the spike protein of the coronavirus mouse hepatitis virus. Experimental introduction of the A528V mutation into the wild-type spike protein resulted in the loss of MAb bindingof the mutant protein, confirming that the single point mutation was responsible for the escape of BCV from immunological selective pressure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 11:43:56