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Titolo:
Butyric acid-induced T-cell apoptosis is mediated by caspase-8 and-9 activation in a Fas-independent manner
Autore:
Kurita-Ochiai, T; Ochiai, K; Fukushima, K;
Indirizzi:
Nihon Univ, Sch Dent, Dept Microbiol, Matsudo, Chiba 2718587, Japan Nihon Univ Matsudo Chiba Japan 2718587 iol, Matsudo, Chiba 2718587, Japan Meikai Univ, Sch Dent, Dept Oral Microbiol, Sakado, Saitama 3500283, JapanMeikai Univ Sakado Saitama Japan 3500283 , Sakado, Saitama 3500283, Japan
Titolo Testata:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
fascicolo: 2, volume: 8, anno: 2001,
pagine: 325 - 332
SICI:
1071-412X(200103)8:2<325:BATAIM>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
DRUG-INDUCED APOPTOSIS; SODIUM-BUTYRATE; DEATH; RECEPTOR; EXPRESSION; REGULATOR; NECROSIS; RELEASE; LIGAND; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Kurita-Ochiai, T Nihon Univ, Sch Dent, Dept Microbiol, Matsudo, Chiba 2718587, Japan Nihon Univ Matsudo Chiba Japan 2718587 ba 2718587, Japan
Citazione:
T. Kurita-Ochiai et al., "Butyric acid-induced T-cell apoptosis is mediated by caspase-8 and-9 activation in a Fas-independent manner", CL DIAG LAB, 8(2), 2001, pp. 325-332

Abstract

Our previous study demonstrated that butyric acid, an extracellular metabolite of periodontopathic bacteria, induced apoptosis in murine thymocytes, splenic T cells, and human Jurkat cells, In this study, we examined whetherCD95 ligand-receptor interaction is involved in butyric acid-induced T-cell apoptosis, Flow cytometry analysis indicated that expression of Fas in Jurkat and T cells from peripheral blood mononuclear cells was not affected by butyric acid treatment, Furthermore, the expression of Fas and FasL protein in Western blotting was not affected by butyric acid treatment. Coincubation with blocking anti-Fas antibodies prevented Fas-induced apoptosis but not butyric acid-induced apoptosis, Anti-Fast antibodies also did not prevent butyric acid-induced apoptosis at any dose examined. Although cytotoxic anti-Fas antibody affected butyric acid-induced apoptosis, a synergistic effect was not seen. Time-dependent activation of caspase-8 and -9 was recognized in butyric acid- as well as Fas-mediated apoptosis, IETD-CHO and LEHD-CHO, specific inhibitors of caspase8 and -9, respectively completely blocked Fas-mediated apoptosis and partially prevented butyric acid induced apoptosis, These results suggest that the Fas-FasL interaction is not involved in butyric acid-induced apoptosis and that caspase-8 and -9-dependent apoptosis plays an important role in butyric acid-induced apoptosis, as well as Fas-induced apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 14:58:36