Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Phosphorylated map kinase (ERK1, ERK2) expression is associated with earlytau deposition in neurones and glial cells, but not with increased nuclearDNA vulnerability and cell death, in Alzheimer disease, Pick's disease, progressive supranuclear palsy and corticobasal degeneration
Autore:
Ferrer, I; Blanco, R; Carmona, M; Ribera, R; Goutan, E; Puig, B; Rey, MJ; Cardozo, A; Vinals, F; Ribalta, T;
Indirizzi:
Univ Barcelona, Hosp Princeps Espanya, Servei Anat Patol, Unitat Neuropatol, Hospitalet De Llobregat 08907, Spain Univ Barcelona Hospitalet De Llobregat Spain 08907 lobregat 08907, Spain Univ Barcelona, Dept Biol Cellular & Anat Patol, E-08007 Barcelona, Spain Univ Barcelona Barcelona Spain E-08007 t Patol, E-08007 Barcelona, Spain Univ Barcelona, Dept Ciencies Fisiol 2, E-08007 Barcelona, Spain Univ Barcelona Barcelona Spain E-08007 isiol 2, E-08007 Barcelona, Spain Univ Barcelona, Hosp Clin, Banc Teixits Neurol, Barcelona, Spain Univ Barcelona Barcelona Spain n, Banc Teixits Neurol, Barcelona, Spain
Titolo Testata:
BRAIN PATHOLOGY
fascicolo: 2, volume: 11, anno: 2001,
pagine: 144 - 158
SICI:
1015-6305(200104)11:2<144:PMK(EE>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; SIGNAL-REGULATED KINASE; AMYLOID-BETA-PROTEIN; HELICAL FILAMENT-TAU; HUMAN BRAIN TAU; NEUROFIBRILLARY TANGLES; PHOSPHATIDYLINOSITOL 3-KINASE; NEUROTROPHIC FACTOR; ABNORMAL PHOSPHORYLATION; NEUROFILAMENT SUBUNIT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
90
Recensione:
Indirizzi per estratti:
Indirizzo: Ferrer, I Univ Barcelona, Hosp Princeps Espanya, Servei Anat Patol, UnitatNeuropatol, Campus Bellvitge, Hospitalet De Llobregat 08907, Spain Univ Barcelona Campus Bellvitge Hospitalet De Llobregat Spain 08907
Citazione:
I. Ferrer et al., "Phosphorylated map kinase (ERK1, ERK2) expression is associated with earlytau deposition in neurones and glial cells, but not with increased nuclearDNA vulnerability and cell death, in Alzheimer disease, Pick's disease, progressive supranuclear palsy and corticobasal degeneration", BRAIN PATH, 11(2), 2001, pp. 144-158

Abstract

Abnormal tau phosphorylation and deposition in neurones and glial cells isone of the major features in taupathies. The present study examines the involvement of the Ras/MEK/ERK pathway of tau phosphorylation in Alzheimer disease (AD), Pick's disease (PiD), progressive supranuclear palsy (PSP) and corticobasal degeneration (CBD), by Western blotting, single and double-labelling immunohistochemistry, and p21Ras activation assay. Since this pathway is also activated in several paradigms of cell death and cell survival, activated ERK expression is also analysed with double-labelling immunohistochemistry and in situ end-labelling of nuclear DMA fragmentation to visualise activated ERK in cells with increased nuclear DNA vulnerability. The MEK1antibody recognises one band of 45 kD that identifies phosphorylation-independent MEK1, whose expression levels are not modified in diseased brains. The ERK antibody recognises one band of 42 kD corresponding to the molecular weight of phosphorylation-independent ERK2; the expression levels, as well as the immunoreactivity of ERK in individual cells, is not changed in AD,PID, PSP and CBD. The antibody MAPK-P distinguishes two bands of 44 kD and42 kD that detect phosphorylated ERK1 and ERK2. MAPK-P expression levels, as seen with Western blotting, are markedly increased in AD, PID, PSP and CBD. Moreover, immunohistochemistry discloses granular precipitates in the cytoplasm of neurones in AD, mainly in a subpopulation of neurones exhibiting early tau deposition, whereas neurones with developed neurofibrillary tangles are less commonly immunostained. MAPK-P also decorates neurones with Pick bodies in PID, early tau deposition in neurones in PSP and CBD, and cortical achromatic neurones in CBD. In addition, strong MAPK-P immunoreactivity is found in large numbers of tau-positive glial cells in PSP and CBD, asseen with double-labelling immunohistochemistry. Yet no co-localisation ofenhanced phosphorylated ERK immunoreactivity and nuclear DNA fragmentationis found in AD, PID, PSP and CBD. Finally, activated Pas expression levelsare increased in AD cases when compared with controls. These results demonstrate increased phosphorylated (active) ERK expression in association withearly tau deposition in neurones and glial cells in taupathies, and suggest activated Ras as the upstream activator of the MEK/ERK pathway of tau phosphorylation in AD.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:06:43