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Titolo:
Transplantation of a fetus with paternal Thy-1(+)CD34(+) cells for chronicgranulomatous disease
Autore:
Muench, MO; Rae, J; Barcena, A; Leemhuis, T; Farrell, J; Humeau, L; Maxwell-Wiggins, JR; Capper, J; Mychaliska, GB; Albanese, CT; Martin, T; Tsukamoto, A; Curnutte, JT; Harrison, MR;
Indirizzi:
Univ Calif San Francisco, Fetal Treatment Ctr, San Francisco, CA 94143 USAUniv Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Immunogenet & Transplantat, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Genentech Inc, Dept Immunol, S San Francisco, CA 94080 USA Genentech Inc S San Francisco CA USA 94080 S San Francisco, CA 94080 USA SyStemix Inc, Palo Alto, CA USA SyStemix Inc Palo Alto CA USASyStemix Inc, Palo Alto, CA USA
Titolo Testata:
BONE MARROW TRANSPLANTATION
fascicolo: 4, volume: 27, anno: 2001,
pagine: 355 - 364
SICI:
0268-3369(200102)27:4<355:TOAFWP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; IN-UTERO TRANSPLANTATION; SEVERE COMBINED IMMUNODEFICIENCY; NATURAL-KILLER-CELLS; FETAL LIVER-CELLS; BONE-MARROW; INUTERO TRANSPLANTATION; T-CELL; PROGENITOR CELLS; ENGRAFTMENT;
Keywords:
in utero transplantation; chronic granulomatous disease; hematopoietic stem cells; Thy-1(+)CD34(+); cells; fetal therapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Muench, MO Univ Calif San Francisco, Fetal Treatment Ctr, 3rd & Parnassus Ave,Room U-442, San Francisco, CA 94143 USA Univ Calif San Francisco 3rd & Parnassus Ave,Room U-442 San Francisco CA USA 94143
Citazione:
M.O. Muench et al., "Transplantation of a fetus with paternal Thy-1(+)CD34(+) cells for chronicgranulomatous disease", BONE MAR TR, 27(4), 2001, pp. 355-364

Abstract

A fetus diagnosed with X-linked chronic granulomatous disease was transplanted with Thy-1(+)CD34(+) cells of paternal origin, The transplant was performed at 14 weeks gestation by ultrasound guided injection into the peritoneal cavity, The fetus was delivered at 38 weeks gestation after an otherwise uneventful pregnancy, Umbilical. cord blood was collected and used to determine the level of peripheral blood chimerism as well as levels of functional engrafted cells. Flow cytometry was used to detect donor leukocytes identified as HLA-A2(-)B7(+) cells, whereas recipient cells were identified asHLA-A2(+)B7(-) cells. No evidence of donor cell engraftment above a level of 0.01% was found. PCR was used to detect HLA-DRB1*15(+) donor cells amongthe recipient's HLA-DRB1*15(-) cells, but no engraftment was seen with a sensitivity of 1:1000. The presence of functional, donor-derived neutrophilswas assessed by flow cytometry using two different fluorescent dyes that measure reactive oxygen species generated by the phagocyte NADPH oxidase, Noevidence of paternal-derived functional neutrophils above a level of 0.15%was observed. Peripheral blood and bone marrow samples were collected at 6months of age. Neither sample showed engraftment by HLA typing using both flow cytometry and PCR, Functional phagocytes were also not observed. Furthermore, no indication of inmunological tolerance specific for the donor cells was indicated by a mixed lymphocyte reaction assay performed at 6 monthsof age. While there appears to be no engraftment of the donor stem cells, the transplant caused no harm to the fetus and the child was healthy at 6 months of age, Analyses of fetal tissues, obtained from elective abortions, revealed that CD3(+) T cells and CD56(+)CD3(-) NK cells are present in the liver at 8 weeks gestation and in the blood by 9 weeks gestation. The presence of these lymphocytes may contribute to the lack of donor cell engraftment in the human fetus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:06:39