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Titolo:
Xenotransplantation of immunodeficient mice with mobilized human blood CD34(+) cells provides an in vivo model for human megakaryocytopoiesis and platelet production
Autore:
Perez, LE; Rinder, HM; Wang, C; Tracey, JB; Maun, N; Krause, DS;
Indirizzi:
Yale Univ, Sch Med, Dept Lab Med, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 d, Dept Lab Med, New Haven, CT 06510 USA Yale Univ, Sch Med, Dept Internal Med, Sect Hematol, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 d, Sect Hematol, New Haven, CT 06510 USA
Titolo Testata:
BLOOD
fascicolo: 6, volume: 97, anno: 2001,
pagine: 1635 - 1643
SICI:
0006-4971(20010315)97:6<1635:XOIMWM>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEMATOPOIETIC-CELLS; HUMAN CORD-BLOOD; IMMUNE-DEFICIENT MICE; RECOMBINANT HUMAN INTERLEUKIN-11; SCID-REPOPULATING CELLS; PERIPHERAL-BLOOD; EX-VIVO; BONE-MARROW; PROGENITOR CELLS; STEM-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Krause, DS Yale Univ, Sch Med, Dept Lab Med, 333 Cedar St,POB 208035, New Haven, CT 06510 USA Yale Univ 333 Cedar St,POB 208035 New Haven CT USA 06510 10 USA
Citazione:
L.E. Perez et al., "Xenotransplantation of immunodeficient mice with mobilized human blood CD34(+) cells provides an in vivo model for human megakaryocytopoiesis and platelet production", BLOOD, 97(6), 2001, pp. 1635-1643

Abstract

The study of megakaryocytopoiesis has been based largely on in vitro assays. We characterize an in vivo model of megakaryocyte and platelet development in which human peripheral blood stem cells (PBSCs) differentiate along megakaryocytic as well as myeloid/lymphoid lineages in sublethally irradiated nonobese diabetic/severe combined immunodeficient (NOD-SCID) mice. Human hematopoiesis preferentially occurs in the bone marrow of the murine recipients, and engraftment is independent of exogenous cytokines, Human colony-forming units-megakaryocyte (CFU-MK) develop pre-dominantly in the bone marrow, and their presence correlates with the overall degree of human cell engraftment. Using a sensitive and specific flow cytometric assay, human platelets are detected in the peripheral blood from weeks 1 to 8 after transplantation. The number of circulating human platelets peaks at week 3 with a mean of 20 x 10(9)/L. These human platelets are functional as assessed by CD62P expression in response to thrombin stimulation in vitro. Exogenous cytokines have a detrimental effect on CFU-MK production after 2 weeks, and animals treated with these cytokines have no circulating platelets 8 weeks after transplantation. Although cytokine stimulation of human PBSCs ex vivo ledto a significant increase in CFU-MK, CD34(+)/41(+), and CD41(+) cells, these ex vivo expanded cells provided only delayed and transient platelet production in vivo, and no CFU-MK developed in vivo after transplantation. In conclusion, xenogeneic transplantation of human PBSCs into NOD/SCID mice provides an excellent in vivo model to study human megakaryocytopoiesis and platelet production. (Blood. 2001;97:1635-1643) (C) 2001 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 09:23:03