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Titolo:
A human hepatoma cell line expressing hepatitis C virus nonstructural proteins tightly regulated by tetracycline
Autore:
Huang, Y; Uchiyama, Y; Fujimura, T; Kanamori, H; Doi, T; Takamizawa, A; Hamakubo, T; Kodama, T;
Indirizzi:
Univ Tokyo, RCAST, Dept Mol Biol & Med, Meguro Ku, Tokyo 1538904, Japan Univ Tokyo Tokyo Japan 1538904 ol & Med, Meguro Ku, Tokyo 1538904, Japan Fujisawa Pharmaceut Co Ltd, Yodogawa Ku, Osaka 5328514, Japan Fujisawa Pharmaceut Co Ltd Osaka Japan 5328514 Ku, Osaka 5328514, Japan Univ Tokyo, Dept Gastroenterol, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 oenterol, Bunkyo Ku, Tokyo 1138655, Japan Osaka Univ, Grad Sch Pharmaceut Sci, Osaka 5650871, Japan Osaka Univ Osaka Japan 5650871 Sch Pharmaceut Sci, Osaka 5650871, Japan Osaka Univ, Res Fdn Microbial Dis, Kagawa 7680061, Japan Osaka Univ Kagawa Japan 7680061 Fdn Microbial Dis, Kagawa 7680061, Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 3, volume: 281, anno: 2001,
pagine: 732 - 740
SICI:
0006-291X(20010302)281:3<732:AHHCLE>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT RNA-POLYMERASE; SERINE PROTEINASE; GENE-EXPRESSION; MAMMALIAN-CELLS; IN-VITRO; NON-A; NS3; IDENTIFICATION; INFECTION; POLYPROTEIN;
Keywords:
hepatitis C virus NS proteins; tetracycline-regulated expression; hepatoma cell lines; DNA microarray;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Kodama, T Univ Tokyo, RCAST, Dept Mol Biol & Med, Meguro Ku, 4-6-1 Komaba,Tokyo 1538904, Japan Univ Tokyo 4-6-1 Komaba Tokyo Japan 1538904 okyo 1538904, Japan
Citazione:
Y. Huang et al., "A human hepatoma cell line expressing hepatitis C virus nonstructural proteins tightly regulated by tetracycline", BIOC BIOP R, 281(3), 2001, pp. 732-740

Abstract

Nonstructural (NS) proteins of hepatitis C virus (HCV) play major roles inviral replication and the pathogenesis of liver diseases. Current studies on antiviral strategies targeting these proteins have been hampered by the lack of efficient cell culture systems. Combining tetracycline-regulated gene expressing system and enhanced green fluorescent protein (EGFP), we generated a human hepatoma cell line inducibly expressing the HCV NS proteins. This cell line exhibited high induction of a full NS transcript (similar to7 kb). In the absence of tetracycline, NS proteins 3, 4A, and 5A of mature sizes were detected by immunoblot analysis and the induction of NS proteins3 to 5B are confirmed by immunofluorescent staining. Using DNA microarray analysis, we characterized the changes in mRNA expression profile of 6416 genes and identified several genes, whose mRNAs are specifically upregulatedby the induction of NS proteins. This cell line provides a unique in vitrohepatoma cell system for the investigation of structural and functional properties of HCV NS proteins. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 11:20:29