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Titolo:
CLONING AND OVEREXPRESSION OF POLYPEPTIDE RELEASE FACTOR-1 OF THERMUS-THERMOPHILUS
Autore:
ITO K; NAKAMURA Y;
Indirizzi:
UNIV TOKYO,INST MED SCI,DEPT TUMOR BIOL,PO TAKANAWA TOKYO 108 JAPAN UNIV TOKYO,INST MED SCI,DEPT TUMOR BIOL TOKYO 108 JAPAN
Titolo Testata:
Biochimie
fascicolo: 5, volume: 79, anno: 1997,
pagine: 287 - 292
SICI:
0300-9084(1997)79:5<287:CAOOPR>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELONGATION-FACTOR-G; ESCHERICHIA-COLI; CRYSTAL-STRUCTURE; GENE; RNA; TERMINATION; STOP; DNA;
Keywords:
TRANSLATION TERMINATION; RELEASE FACTOR; THERMUS THERMOPHILUS; RF1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
K. Ito e Y. Nakamura, "CLONING AND OVEREXPRESSION OF POLYPEPTIDE RELEASE FACTOR-1 OF THERMUS-THERMOPHILUS", Biochimie, 79(5), 1997, pp. 287-292

Abstract

A prfA gene encoding polypeptide release factor RFI was cloned from Thermus thermophilus. T thermophilus RFI shares 68% homology with Escherichia coil RF1, and its overproduction reduced readthrough translation of UAG, not of UGA, in the lacZ gene. Rapid purification of T thermophilus RF1 was achieved by T7-RNA polymerase driven overexpression of T thermophilus RF1 protein with a C-terminal histidine tag.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 07:40:52