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Titolo:
Improved intracellular delivery of oligonucleotides by square wave electroporation
Autore:
Liu, YQ; Bergan, R;
Indirizzi:
Northwestern Univ, Sch Med, Dept Med, Div Hematol Oncol, Chicago, IL 60611USA Northwestern Univ Chicago IL USA 60611 ematol Oncol, Chicago, IL 60611USA Northwestern Univ, Robert H Lurie Canc Ctr, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 ie Canc Ctr, Chicago, IL 60611 USA
Titolo Testata:
ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT
fascicolo: 1, volume: 11, anno: 2001,
pagine: 7 - 14
SICI:
1087-2906(200102)11:1<7:IIDOOB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE KINASE-ACTIVITY; PROSTATE-CANCER CELLS; FOCAL ADHESION KINASE; C-MYC; ANTISENSE OLIGONUCLEOTIDES; GENE-TRANSFER; GROWTH-INHIBITION; LYMPHOMA GROWTH; LEUKEMIA-CELLS; OLIGODEOXYNUCLEOTIDES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Bergan, R Northwestern Univ, Sch Med, Dept Med, Div Hematol Oncol, Olson Pavil 8524,710 N Fairbanks, Chicago, IL 60611 USA Northwestern Univ Olson Pavil 8524,710 N Fairbanks Chicago IL USA 60611
Citazione:
Y.Q. Liu e R. Bergan, "Improved intracellular delivery of oligonucleotides by square wave electroporation", ANTISENSE N, 11(1), 2001, pp. 7-14

Abstract

Prior studies have shown that electroporation is a simple and effective method for the introduction of oligonucleotides (ODN) into cells. In ex vivo bone marrow purging models, electroporation of ODN into cells has been associated with selective killing of human neoplastic cells while sparing hematopoietic stem cells. Prior studies used conventional electroporation methods (i.e., exponential decay) to introduce ODN into cells. Square wave electroporation allows the delivery of a more defined and regulated electrical pulse and is associated with high transfection efficiencies in a variety of systems. The current study was undertaken to determine whether square wave electroporation was more effective than exponential decay electroporation for the delivery of ODN into hematopoietic cells, Using fluorescein-tagged ODN and K562, chronic myelogenous leukemia (CR IL) cells, higher transfectionrates were observed after square wave electroporation, In addition, c-myc antisense ODN were more effective in reducing c-myc protein when introducedby square wave electroporation, as compared with introduction by exponential decay electroporation, Square wave electroporation is thus identified asthe optimal method for delivering ODN into hematopoietic cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:18:18