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Titolo:
Heterogeneous nuclear ribonucleoprotein C binds exclusively to the functionally important UUUUU-motifs in the human papillomavirus type-1 AU-rich inhibitory element
Autore:
Sokolowski, M; Schwartz, S;
Indirizzi:
Univ Uppsala, Ctr Biomed, Dept Med Biochem & Microbiol, S-75123 Uppsala, Sweden Univ Uppsala Uppsala Sweden S-75123 & Microbiol, S-75123 Uppsala, Sweden
Titolo Testata:
VIRUS RESEARCH
fascicolo: 2, volume: 73, anno: 2001,
pagine: 163 - 175
SICI:
0168-1702(200103)73:2<163:HNRCBE>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
3' UNTRANSLATED REGION; LATE MESSENGER-RNAS; HUMAN-IMMUNODEFICIENCY-VIRUS; NEGATIVE REGULATORY ELEMENT; 3'-UNTRANSLATED REGION; PROTEIN TETRAMER; CELLULAR FACTORS; REV PROTEIN; HNRNP; SEQUENCES;
Keywords:
human papillomavirus-1; AU-rich element; 3 ' UTR; heterogeneous nuclear ribonucleoprotein C; HuR; AU-rich RNA binding factor 1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Schwartz, S Univ Uppsala, Ctr Biomed, Dept Med Biochem & Microbiol, Husargatan 3,Box 582, S-75123 Uppsala, Sweden Univ Uppsala Husargatan 3,Box 582 Uppsala Sweden S-75123 eden
Citazione:
M. Sokolowski e S. Schwartz, "Heterogeneous nuclear ribonucleoprotein C binds exclusively to the functionally important UUUUU-motifs in the human papillomavirus type-1 AU-rich inhibitory element", VIRUS RES, 73(2), 2001, pp. 163-175

Abstract

We have previously identified an inhibitory, 57 nt AU-rich sequence in theHPV-I late 3' UTR, termed as the HPV-I AU-rich element (h1ARE). It contains two types of functionally important motifs: two AUUUA sequences and threeUUUUU sequences. We have shown that the h1ARE interacts with heterogeneousnuclear ribonucleoprotein (hnRNP) C1/C2 and the ELAV-like HuR protein. While we have shown that HuR binds to both the AUUUA- and the UUUUU-motifs. the interaction between hnRNP C and the h1ARE has not been investigated in detail. Here, we have used recombinant (r)hnRNP C1 to study the interaction between hnRNP C1 and the h1ARE by using the UV cross-linking assay. We demonstrate that (r)hnRNP C1 cross-links specifically to the three functionally important UUUUU-motifs in the h1ARE. In contrast. (r)hnRNP does not UV cross-link to the functionally important AUUUA-motifs in the h1ARE. Conclusively, the binding ability of hnRNP C to the h1ARE correlates with its partially inhibitory function. Additionally, the recombinant AU-rich RNA binding factor 1 (AUF1) was analyzed for binding to the h1ARE by using the UV cross-linking assay, but the results revealed no specificity for the functionally important AUUUA- and UUUUU-motifs. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:41:48