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Titolo:
Early detection of radiation-induced glomerular injury by albumin permeability assay
Autore:
Sharma, M; Sharma, R; Ge, XL; Fish, BL; McCarthy, ET; Savin, VJ; Cohen, EP; Moulder, JE;
Indirizzi:
Med Coll Wisconsin, MEB, CVRC, Dept Med, Milwaukee, WI 53226 USA Med Coll Wisconsin Milwaukee WI USA 53226 pt Med, Milwaukee, WI 53226 USA Med Coll Wisconsin, Dept Radiat Oncol, Milwaukee, WI 53226 USA Med Coll Wisconsin Milwaukee WI USA 53226 Oncol, Milwaukee, WI 53226 USA
Titolo Testata:
RADIATION RESEARCH
fascicolo: 3, volume: 155, anno: 2001,
pagine: 474 - 480
SICI:
0033-7587(200103)155:3<474:EDORGI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED RAT GLOMERULI; MARROW TRANSPLANT NEPHROPATHY; TOTAL-BODY IRRADIATION; CAPILLARY WALL; MORPHOMETRIC ANALYSIS; TUBULAR ALTERATIONS; PERMSELECTIVITY; MACROMOLECULES; INCREASES; SUPEROXIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Sharma, M Med Coll Wisconsin, MEB, CVRC, Dept Med, Rm M4040,8701 WatertownPlank Rd,Milwaukee, WI 53226 USA Med Coll Wisconsin Rm M4040,8701 Watertown Plank Rd Milwaukee WI USA 53226
Citazione:
M. Sharma et al., "Early detection of radiation-induced glomerular injury by albumin permeability assay", RADIAT RES, 155(3), 2001, pp. 474-480

Abstract

Renal irradiation leads predictably to glomerular vascular injury, cell lysis, matrix accumulation, sclerosis and loss of renal function. The immediate effects of renal irradiation that may be associated with glomerular pathology and proteinuria are not clear in the human disease or its rat model. We hypothesized that radiation-induced injury causes immediate and subtle alterations in glomerular physiology independent of the neurohumoral and hemodynamic regulatory mechanisms. We employed a sensitive iii vitro functional assay of glomerular albumin permeability (P-alb) to demonstrate radiation-induced damage to the glomerular filtration barrier immediately after total-body irradiation of rats. In blinded experiments, control rats were sham-treated, and experimental rats received 9.5 Gy X rays. Rats were killed humanely at 1 h to 9 weeks after irradiation and glomeruli were isolated. In parallel experiments, glomeruli were isolated from normal rats and irradiated in vitro. The change in glomerular capillary permeability due to an experimental oncotic gradient was determined using videomicroscopy and P-alb wascalculated. Results show that in vivo or kt vitro irradiation of glomerulicaused an increased P-alb at 1 h. Increased P-alb was observed up to 3 weeks after irradiation. Glomeruli from mice irradiated with 9.5 or 19.0 Gy X rays did not show increased P-alb at 1 h postirradiation, We conclude that glomerular protein permeability of irradiated rats increases in a dose-dependent manner immediately after irradiation and that it appears to be independent of hemodynamic or systemic influences. (C) 2001 by Radiation ResearchSociety.

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Documento generato il 26/09/20 alle ore 04:56:40