Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Transforming growth factor beta in the human prostate: Its role in stromal-epithelial interactions in non-cancerous cell culture
Autore:
Blanchere, M; Mestayer, C; Saunier, E; Broshuis, M; Mowszowicz, I;
Indirizzi:
Fac Med Necker Enfants Malad, Serv Endocrinol & Med Reprod, Lab Rech Physiol & Pathol Gonad, F-75743 Paris, France Fac Med Necker Enfants Malad Paris France F-75743 F-75743 Paris, France Fac Med Pitie Salpetriere, Serv Biochim Med, Paris, France Fac Med Pitie Salpetriere Paris France Serv Biochim Med, Paris, France
Titolo Testata:
PROSTATE
fascicolo: 4, volume: 46, anno: 2001,
pagine: 311 - 318
SICI:
0270-4137(20010301)46:4<311:TGFBIT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
TGF-BETA; EXTRACELLULAR-MATRIX; HORMONAL-REGULATION; EXPRESSION; FIBROBLAST; LINES; GENE; PROLIFERATION; TGF-BETA-1; RECEPTORS;
Keywords:
stromal-epithelial interactions; prostate; TGF beta; cell growth;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Mowszowicz, I Fac Med Necker Enfants Malad, Serv Endocrinol & Med Reprod, Lab Rech Physiol & Pathol Gonad, 156 Rue Vaugirard, F-75743 Paris, France Fac Med Necker Enfants Malad 156 Rue Vaugirard Paris France F-75743
Citazione:
M. Blanchere et al., "Transforming growth factor beta in the human prostate: Its role in stromal-epithelial interactions in non-cancerous cell culture", PROSTATE, 46(4), 2001, pp. 311-318

Abstract

BACKGROUND. Stromal-epithelial interactions play a critical role in prostate development, but the precise mechanisms are still unknown. Transforming growth factor-beta (TGF beta) could be a potential mediator of these interactions, but there is as yet no clear demonstration of its role. METHODS. Separate cultures and co-cultures of fibroblasts and epithelial human prostate cells were performed. We measured TGF beta1 and TGF beta2 secretion by specific ELISA assay, cell growth by DNA assay, and TGF beta typeII receptor expression by RT-PCR. RESULTS. Go-culture resulted in a 20% inhibition of epithelial cell growth, similar to that obtained after TGF beta treatment (2 ng/ml for 48 hr), but without affecting fibroblast proliferation. This was accompanied by a five- to six-fold increase in epithelial TGF beta2 secretion. CONCLUSIONS. These results demonstrate for the first time that TGF beta2 secretion by prostate epithelial cells is under the direct control of a diffusible factor secreted by fibroblasts. They emphasize the role of TGF beta in stromal-epithelial interactions. Prostate 46:311-318, 2001. (C) 2001 Wiley-Liss,Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:38:19