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Titolo:
beta-Adrenergic stimulation of volume-sensitive chloride transport in lamprey erythrocytes
Autore:
Nikinmaa, M; Salama, A; Bogdanova, A; Virkki, LV;
Indirizzi:
Univ Turku, Dept Biol, FIN-20014 Turku, Finland Univ Turku Turku FinlandFIN-20014 , Dept Biol, FIN-20014 Turku, Finland Univ Helsinki, Dept Biosci, Div Anim Physiol, FIN-00014 Helsinki, Finland Univ Helsinki Helsinki Finland FIN-00014 ol, FIN-00014 Helsinki, Finland
Titolo Testata:
PHYSIOLOGICAL AND BIOCHEMICAL ZOOLOGY
fascicolo: 1, volume: 74, anno: 2001,
pagine: 45 - 51
SICI:
1522-2152(200101/02)74:1<45:BSOVCT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
RED-BLOOD-CELLS; LAMPETRA-FLUVIATILIS ERYTHROCYTES; CANINE ATRIAL CELLS; PETROMYZON-MARINUS; MYXINE-GLUTINOSA; MEMBRANE-TRANSPORT; OXYGEN-AFFINITY; ANION MOVEMENTS; TAURINE EFFLUX; ION-TRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Nikinmaa, M Univ Turku, Dept Biol, FIN-20014 Turku, Finland Univ Turku Turku Finland FIN-20014 FIN-20014 Turku, Finland
Citazione:
M. Nikinmaa et al., "beta-Adrenergic stimulation of volume-sensitive chloride transport in lamprey erythrocytes", PHYSIOL B Z, 74(1), 2001, pp. 45-51

Abstract

We measured the effects of a beta -adrenergic agonist, isoproterenol, on chloride transport and volume regulation of lamprey (Lampetra fluviatilis) erythrocytes in isotonic (288 mosm L-1) and hypotonic (192 mosm L-1) medium. Isoproterenol at a high concentration (10(-5) M) did not influence chloride transport in isotonic medium but markedly increased chloride fluxes in hypotonic conditions: unidirectional flux increased from 100 mmol kg dcw(-1) h(-1) in the absence to 350 mmol kg dcw(-1) h(-1) (dcw = dry cell weight) in the presence of isoproterenol. Simultaneously, the half-time for volume recovery decreased from 27 to 9 min. Isoproterenol caused an increase in cellular cyclic AMP (cAMP) concentration. The stimulation of chloride transport in hypotonic conditions could be induced by application of the permeable cAMP analogue, 8-bromo-cyclicAMP, suggesting that the effect of beta -adrenergic stimulation on chloride transport occurs downstream of cAMP production. As isoproterenol did not affect unidirectional rubidium fluxes in hypotonic conditions, the transport pathway influenced by beta -adrenergic stimulation is most likely the swelling-activated chloride channel. Because the beta -adrenergic agonist only influenced the transport in hypotonic conditions despite the fact that cAMP concentration also increased in isotonic conditions, the activation may involve a volume-dependent conformational changein the chloride channel.

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Documento generato il 04/04/20 alle ore 08:44:18