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Titolo:
Transcriptional activation by hepatocyte nuclear factor-4 in a cell-free system derived from rat liver nuclei
Autore:
Harish, S; Khanam, T; Mani, S; Rangarajan, P;
Indirizzi:
Indian Inst Sci, Dept Biochem, Bangalore 560012, Karnataka, India Indian Inst Sci Bangalore Karnataka India 560012 560012, Karnataka, India
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 5, volume: 29, anno: 2001,
pagine: 1047 - 1053
SICI:
0305-1048(20010301)29:5<1047:TABHNF>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORMONE RECEPTOR SUPERFAMILY; 9-CIS RETINOIC ACID; BINDING PROTEIN; MOLECULAR-BIOLOGY; GENE-EXPRESSION; RNA-POLYMERASE; DIRECT REPEAT; HNF-4; PROMOTER; RXR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Rangarajan, P Indian Inst Sci, Dept Biochem, Bangalore 560012, Karnataka, India Indian Inst Sci Bangalore Karnataka India 560012 aka, India
Citazione:
S. Harish et al., "Transcriptional activation by hepatocyte nuclear factor-4 in a cell-free system derived from rat liver nuclei", NUCL ACID R, 29(5), 2001, pp. 1047-1053

Abstract

Hepatocyte nuclear factor-4 (HNF4) regulates gene expression by binding todirect repeat motifs of the RG(G/T)TCA sequence separated by one nucleotide (DR1), In this study we demonstrate that endogenous HNF4 present in rat liver nuclear extracts, as well as purified recombinant HNF4, activates transcription from naked DNA templates containing multiple copies of the DR1 element linked to the adenovirus major late promoter. Recombinant HNF4 also activates transcription from the rat cellular retinol binding protein II (CRBPII) promoter in vitro. The region between -105 and -63 bp of this promoter is essential for HNF-mediated transactivation. The addition of a peptide containing the LXXLL motif abolished HNF4-mediated transactivation in vitrosuggesting that LXXLL-containing protein factor(s) are involved in HNF4-mediated transactivation in rat liver nuclear extracts, This is the first report on transactivation by HNF4 in a cell-free system derived from rat livernuclei.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 23:05:00