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Titolo:
Profiling and verification of gene expression patterns in normal and malignant human prostate tissues by cDNA microarray analysis
Autore:
Chaib, H; Cockrell, EK; Rubin, MA; Macoska, JA;
Indirizzi:
Univ Michigan, Dept Surg, Urol Sect, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 , Urol Sect, Ann Arbor, MI 48109 USA Univ Michigan, Dept Pathol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 Dept Pathol, Ann Arbor, MI 48109 USA Univ Michigan, Ctr Comprehens Canc, Ann Arbor, MI 48109 USA Univ MichiganAnn Arbor MI USA 48109 rehens Canc, Ann Arbor, MI 48109 USA
Titolo Testata:
NEOPLASIA
fascicolo: 1, volume: 3, anno: 2001,
pagine: 43 - 52
SICI:
1522-8002(200101/02)3:1<43:PAVOGE>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; EPITHELIAL-CELLS; TRANSFORMING GROWTH-FACTOR-BETA-1; MICROSATELLITE INSTABILITY; CARCINOMA-CELLS; DNA; PROTEINS; CANCER; REPAIR; HYPERMETHYLATION;
Keywords:
microarray; prostate; expression; RNA; cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Macoska, JA Univ Michigan, Dept Surg, Urol Sect, 7306 CCGC,1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA Univ Michigan 7306 CCGC,1500 E Med Ctr Dr Ann Arbor MI USA 48109
Citazione:
H. Chaib et al., "Profiling and verification of gene expression patterns in normal and malignant human prostate tissues by cDNA microarray analysis", NEOPLASIA, 3(1), 2001, pp. 43-52

Abstract

cDNA microarray technology allows the "profiling" of gene expression patterns for virtually any cellular material. In this study, we applied cDNA microarray technology to profile changes in gene expression associated with human prostate tumorigenesis. RNA prepared from normal and malignant prostatetissue was examined for the expression levels of 588 human genes. Four different methods for data normalization were utilized. Of these, normalization to ACTB expression proved to be the most rigorous technique with the least probability of producing spurious results. After normalization to ACTB expression, 15 of 588 (2.6%) genes examined by array analysis were differentially expressed by a factor of 2x or more in malignant compared to normal prostate tissues. The expression patterns for 8 of 15 genes have been reported previously in prostate tissues (TGF beta3, TGFBR3, IGFII, IGFBP2, VEGF, FGF7, ERBB3, MYC), but those of seven genes are reported here for the first time (MLH1, CYP1B1, RFC4, EPHB3, MGST1, BTEB2, MLP). These genes describe at least four metabolic and signaling pathways likely disrupted in human prostate tumorigenesis. Reverse transcriptase polymerase chain reaction (RT-PCR) and Northern blot analyses quantitated with reference to ACTB expressionlevels verified the trends in gene expression levels observed by array analysis for 14/15 and 8/8 genes, respectively. However, RT-PCR and Northern blot analyses accurately verified the "fold" differences in expression levels for only 6/15 (40%) and 7/8 (88%) of genes examined, respectively, demonstrating the need to better validate quantitative differences in gene expression revealed by array-based techniques.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 00:36:31