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Titolo:
Sequential-replenishment mechanism of exocytosis in pancreatic acini
Autore:
Nemoto, T; Kimura, R; Ito, K; Tachikawa, A; Miyashita, Y; Iino, M; Kasai, H;
Indirizzi:
Natl Inst Physiol Sci, Dept Cell Physiol, Okazaki, Aichi 4448585, Japan Natl Inst Physiol Sci Okazaki Aichi Japan 4448585 i, Aichi 4448585, Japan Univ Tokyo, Sch Med, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Sch Med, Bunkyo Ku, Tokyo 1130033, Japan Japan Sci & Technol Corp, CREST, Bunkyo Ku, Tokyo 1130033, Japan Japan Sci& Technol Corp Tokyo Japan 1130033 yo Ku, Tokyo 1130033, Japan
Titolo Testata:
NATURE CELL BIOLOGY
fascicolo: 3, volume: 3, anno: 2001,
pagine: 253 - 258
SICI:
1465-7392(200103)3:3<253:SMOEIP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CA2+ SPIKES; INOSITOL TRISPHOSPHATE; REGULATED EXOCYTOSIS; CELLS; CALCIUM; FUSION; MICROSCOPY; SECRETION; GRANULES; RELEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Kasai, H Natl Inst Physiol Sci, Dept Cell Physiol, Okazaki, Aichi 4448585,Japan Natl Inst Physiol Sci Okazaki Aichi Japan 4448585 4448585, Japan
Citazione:
T. Nemoto et al., "Sequential-replenishment mechanism of exocytosis in pancreatic acini", NAT CELL BI, 3(3), 2001, pp. 253-258

Abstract

Here we report exocytosis of zymogen granules, as examined by multiphoton excitation imaging in intact pancreatic acini, Cholecystokinin induces Ca2oscillations that trigger exocytosis when the cytosolic Ca2+ concentrationexceeds 1 muM. Zymogen granules fused with the plasma membrane maintain their Omega -shaped profile for an average of 220 s and serve as targets for sequential fusion of granules that are located within deeper layers of the cell. This secondary exocytosis occurrs as rapidly as the primary exocytosis and accounts for most exocytotic events. Granule-granule fusion does not seem to precede primary exocytosis, indicating that secondary fusion eventsmay require a plasma-membrane factor, This sequential-replenishment mechanism of exocytosis allows the cell to take advantage of a large supply of fusion-ready granules without needing to transport them to the plasma membrane.

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Documento generato il 23/01/21 alle ore 10:20:34