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Titolo:
Lactose supports Muller cell protein expression patterns in the absence ofthe retinal pigment epithelium
Autore:
Jablonski, M; Iannaccone, A;
Indirizzi:
Univ Tennessee, Ctr Hlth Sci, Dept Ophthalmol, Retinal Degenerat Res Ctr, Memphis, TN 38163 USA Univ Tennessee Memphis TN USA 38163 enerat Res Ctr, Memphis, TN 38163 USA
Titolo Testata:
MOLECULAR VISION
fascicolo: 5, volume: 7, anno: 2001,
pagine: 27 - 35
SICI:
1090-0535(20010213)7:5<27:LSMCPE>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBRILLARY ACIDIC PROTEIN; GLUTAMINE-SYNTHETASE EXPRESSION; FIBROBLAST GROWTH-FACTOR; EMBRYONIC NEURAL RETINA; RAT RETINA; PHOTORECEPTOR DEGENERATION; BINDING PROTEINS; LIGHT DAMAGE; GLIAL CELLS; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Jablonski, M Univ Tennessee, Ctr Hlth Sci, Dept Ophthalmol, Retinal Degenerat Res Ctr, Memphis, TN 38163 USA Univ Tennessee Memphis TN USA 38163 r, Memphis, TN 38163 USA
Citazione:
M. Jablonski e A. Iannaccone, "Lactose supports Muller cell protein expression patterns in the absence ofthe retinal pigment epithelium", MOL VIS, 7(5), 2001, pp. 27-35

Abstract

PURPOSE: We have previously shown that lactose, but not mannose, promotes the assembly of nascent photoreceptor outer segments in the absence of the retinal pigment epithelium (RPE). The purpose of the present study was to determine if, in addition to the improved outer segment assembly observed inthe presence of lactose, biosynthetic changes in Muller cells could also be detected. METHODS: The RPE was removed from intact isolated Xenopus embryonic eyes that were allowed to complete differentiation in Niu-Twitty medium, Niu-Twitty with mannose, or Niu-Twitty with lactose. Control retinas matured in vitro with an adherent RPE. Retinal morphology was evaluated for organized folding of outer segment membranes and cell loss. In addition, the expression of three Muller cell proteins, glial fibrillary acidic protein (GFAP), cellular retinaldehyde-binding protein (CRALBP), and glutamine synthetase, was examined. RESULTS: In control retinas, GFAP is undetectable, CRALBP heavily immunolabels Muller cells, and radial patterns of glutamine synthetase immunoreactivity are present. In the absence of the RPE, Muller cells upregulate GFAP expression, CRALBP labeling is present at a slightly reduced level, and glutamine synthetase immunolabeling is negligible. Neither mannose nor lactose modify significantly the expression of CRALBP. Similarly, both compounds completely prevent the upregulation of GFAP. However, normal glutamine synthetase expression was observed only in the presence of lactose, but not in the presence of mannose. Statistical analyses of slot blot-based protein quantification confirmed our immunochemical results. CONCLUSIONS: In RPE-deprived retinas supplemented with lactose, Muller cells were morphologically normal. The proper photoreceptor outer segment morphogenesis observed under these conditions was uniquely associated with normal levels of glutamine synthetase expression. The exact significance of this finding with respect to photoreceptor outer segment morphogenesis is unknown. We suggest that glutamine synthetase may be a marker of Muller cell metabolic or structural integrity that may reflect the enhanced ability of these cells, in the presence of lactose, to support photoreceptor outer segment morphogenesis.

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Documento generato il 26/01/20 alle ore 22:21:12