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Titolo:
Cutaneous transfection and immune responses to intradermal nucleic acid vaccination are significantly enhanced by in vivo electropermeabilization
Autore:
Drabick, JJ; Glasspool-Malone, J; Somiari, S; King, A; Malone, RW;
Indirizzi:
MIST Inst, Rockville, MD 20850 USA MIST Inst Rockville MD USA 20850MIST Inst, Rockville, MD 20850 USA Walter Reed Army Med Ctr, Hematol Oncol Serv, Washington, DC 20307 USA Walter Reed Army Med Ctr Washington DC USA 20307 Washington, DC 20307 USA CytoPulse Sci, Hanover, MD 21045 USA CytoPulse Sci Hanover MD USA 21045CytoPulse Sci, Hanover, MD 21045 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 2, volume: 3, anno: 2001,
pagine: 249 - 255
SICI:
1525-0016(200102)3:2<249:CTAIRT>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
B SURFACE-ANTIGEN; DNA-MEDIATED IMMUNIZATION; GENE-TRANSFER; IN-VIVO; DENDRITIC CELLS; PLASMID DNA; HEPATITIS; DELIVERY; ELECTROPORATION; SKIN;
Keywords:
gene therapy; nucleic acid vaccination; electroporation; electropermeabilization; skin; dendritic cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Malone, RW MIST Inst, 308 Carr Ave, Rockville, MD 20850 USA MIST Inst 308 Carr Ave Rockville MD USA 20850 lle, MD 20850 USA
Citazione:
J.J. Drabick et al., "Cutaneous transfection and immune responses to intradermal nucleic acid vaccination are significantly enhanced by in vivo electropermeabilization", MOL THER, 3(2), 2001, pp. 249-255

Abstract

Naked DNA injection with electropermeabilization (EP) is a promising method for nucleic acid vaccination (NAV) and in vivo gene therapy. Skin is an ideal target for NAV due to ease of administration and the accessibility of large numbers of antigen-presenting cells within the tissue. This study demonstrates that in vivo skin EP may be used to increase transgene expressionup to an average of 83-fold relative to naked DNA injection (50 mug DNA per dose, P < 0.005). Transfected cells were principally located in dermis and included adipocytes, fibroblasts, endothelial cells, and numerous mononuclear cells with dendritic processes in a porcine model. Transfected cells were also observed in lymph nodes draining electropermeabilized sites. A HBVsAg-coding plasmid was used to test skin EP-mediated NAV in a murine model. Analysis of humoral immune responses including immunoglobulin subclass profiles revealed strong enhancement of EP-mediated NAV relative to naked DNAinjection, with a Th1-dominant, mixed-response pattern compared to immunization with HBV sAg protein that was exclusively Th2 (P = 0.02). Applications for these findings include NAV-based modulation of immune responses to pathogens, allergens, and tumor-associated antigens and the modification of tolerance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/02/20 alle ore 16:35:48