Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
AAV-mediated delivery of ciliary neurotrophic factor prolongs photoreceptor survival in the rhodopsin knockout mouse
Autore:
Liang, FQ; Dejneka, NS; Cohen, DR; Krasnoperova, NV; Lem, J; Maguire, AM; Dudus, L; Fisher, KJ; Bennett, J;
Indirizzi:
Univ Penn, Scheie Eye Inst, Stellar Chance Labs 310, FM Kirby Ctr Mol Ophthalmol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 phthalmol, Philadelphia, PA 19104 USA New England Med Ctr, Dept Ophthalmol, Boston, MA 02111 USA New England MedCtr Boston MA USA 02111 Ophthalmol, Boston, MA 02111 USA Tulane Univ, Med Ctr, Dept Pathol, New Orleans, LA 70112 USA Tulane Univ New Orleans LA USA 70112 pt Pathol, New Orleans, LA 70112 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 2, volume: 3, anno: 2001,
pagine: 241 - 248
SICI:
1525-0016(200102)3:2<241:ADOCNF>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT ADENOASSOCIATED VIRUS; RETINAL DEGENERATION; RETINITIS-PIGMENTOSA; ROD PHOTORECEPTORS; GENE-TRANSFER; IN-VIVO; TRANSGENE EXPRESSION; GROWTH-FACTORS; NULL MUTATION; RD/RD MOUSE;
Keywords:
CNTF; AAV; photoreceptor; apoptosis; retinal degeneration; gene therapy; neurotrophic factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Bennett, J Univ Penn, Scheie Eye Inst, Stellar Chance Labs 310, FM Kirby Ctr Mol Ophthalmol, 422 Curie Blvd, Philadelphia, PA 19104 USA Univ Penn 422Curie Blvd Philadelphia PA USA 19104 PA 19104 USA
Citazione:
F.Q. Liang et al., "AAV-mediated delivery of ciliary neurotrophic factor prolongs photoreceptor survival in the rhodopsin knockout mouse", MOL THER, 3(2), 2001, pp. 241-248

Abstract

Retinitis pigmentosa (RP), an inherited retinal degenerative disease causing blindness, is characterized by progressive apoptotic death of photoreceptors. Therapeutic modification of photoreceptor apoptosis may provide an effective therapy for this disorder. Ciliary neurotrophic factor (CNTF) has been shown to promote survival of a number of different neuronal cell types,including photoreceptors. The present study aimed to test whether adeno-associated virus (AAV)-mediated delivery of the gene encoding CNTF delays photoreceptor death in the rhodopsin knockout (opsin(-/-)) mouse, an animal model of RP. The vector was made to express a secretable form of CNTF in tandem with a marker GFP. Cultured 293 cells transduced with this virus expressed both CNTF and GFP. The conditioned media from such cells supported the survival of chick dorsal root ganglion neurons in the same manner as recombinant CNTF. Subretinal administration of this virus led to efficient transduction of photoreceptors as indicated by GFP fluorescence and CNTF immunostaining. Histologic examination showed significant photoreceptor preservationin the injected quadrant of the retina. This protection lasted through termination of the experiment (3 months). AAV-mediated delivery of CNTF may have implications for the treatment of human retinal degeneration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 06:18:06