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Titolo:
Membrane macrophage colony-stimulating factor on MADB106 breast cancer cells does not activate cytotoxic macrophages but immunizes rats against breast cancer
Autore:
Williams, CC; Trinh, H; Tran, TV; Dan, QH; Sanchez, R; Delgado, C; Chen, YJ; Sippel, B; Jeffes, EWB; Wepsic, HT; Jadus, MR;
Indirizzi:
Vet Affairs Med Ctr, Lab Serv, Long Beach, CA 90822 USA Vet Affairs Med Ctr Long Beach CA USA 90822 erv, Long Beach, CA 90822 USA Univ Calif Irvine, Dept Pathol, Irvine, CA 92117 USA Univ Calif Irvine Irvine CA USA 92117 , Dept Pathol, Irvine, CA 92117 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 2, volume: 3, anno: 2001,
pagine: 216 - 224
SICI:
1525-0016(200102)3:2<216:MMCFOM>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; MESSENGER-RNA; IN-VIVO; M-CSF; TUMORICIDAL ACTIVITY; GENE-EXPRESSION; HUMAN-OVARIAN; KILLER-CELLS; TUMOR-CELLS; ANTIBODY;
Keywords:
macrophage colony-stimulating factor; macrophages; breast cancer; tumor immunity; tumor vaccine; cisplatin; taxol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Jadus, MR Vet Affairs Med Ctr, Lab Serv, Box 113,5901 E 7th St, Long Beach, CA 90822USA Vet Affairs Med Ctr Box 113,5901 E 7th St Long Beach CA USA 90822
Citazione:
C.C. Williams et al., "Membrane macrophage colony-stimulating factor on MADB106 breast cancer cells does not activate cytotoxic macrophages but immunizes rats against breast cancer", MOL THER, 3(2), 2001, pp. 216-224

Abstract

Weakly immunogenic, but highly malignant, rat MADB106 breast cancer cells were retrovirally transduced with the membrane form of macrophage colony-stimulating factor (mM-CSF). The cloned mM-CSF-transfected MADB106 cells physically conjugated with macrophages, but were not killed by the macrophages in 48-h cytotoxicity assays. Macrophages killed the mM-CSF-expressing tumors in the presence of noncytotoxic doses of either taxol or taxol plus cisplatin. This indicated that macrophages bind to the mM-CSF expressed on the tumor cells, but for successful macrophage cytotoxicity to occur against mM-CSF-transduced tumor cells other factors must be present. The mM-CSF-transfected tumor cells were rejected when inoculated subcutaneously into normal rats. Cloned MADB106 tumor cells which expressed high amount of mM-CSF wererejected, while tumor cells that displayed lower levels of mM-CSF grew in 60% of the inoculated rats. The mM-CSF-transfected tumors that grew were smaller and had a greater amount of necrosis, compared to the viral vector tumors. Rats that spontaneously rejected the mM-CSF-transfected MADB106 cellsshowed rechallenge resistance to unmodified parental MADB106 and R3230Ac breast cancers, but not to the F98 glioma. These observations suggest that breast cancer-specific immunity was induced by the inoculation of mM-CSF-expressing MADB106 tumor cells.

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Documento generato il 26/01/20 alle ore 10:21:31