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Titolo:
Soluble P-selectin antagonist mediates rolling velocity and adhesion of leukocytes in acutely inflamed venules
Autore:
Eppihimer, MJ; Schaub, RG;
Indirizzi:
Wyeth Genet Inst Inc, Discovery Res Immunol & Hemostasis, Andover, MA 01810 USA Wyeth Genet Inst Inc Andover MA USA 01810 mostasis, Andover, MA 01810 USA
Titolo Testata:
MICROCIRCULATION
fascicolo: 1, volume: 8, anno: 2001,
pagine: 15 - 24
SICI:
1073-9688(200102)8:1<15:SPAMRV>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECRUITMENT IN-VIVO; GLYCOPROTEIN LIGAND-1; REPERFUSION INJURY; CELL-ADHESION; ISCHEMIA/REPERFUSION; ENDOTHELIUM; MICE; EXPRESSION; MECHANISMS; MOLECULE-1;
Keywords:
endothelial cell; intravital microscopy; leukocyte; PSGL-1; selectin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Eppihimer, MJ Wyeth Genet Inst Inc, Discovery Res Immunol & Hemostasis, 1 Burtt Rd, Andover, MA 01810 USA Wyeth Genet Inst Inc 1 Burtt Rd Andover MA USA 01810 810 USA
Citazione:
M.J. Eppihimer e R.G. Schaub, "Soluble P-selectin antagonist mediates rolling velocity and adhesion of leukocytes in acutely inflamed venules", MICROCIRCUL, 8(1), 2001, pp. 15-24

Abstract

Objective: Leukocyte rolling is recognized as an important event in facilitating the extravasation of leukocytes from the vascular to the interstitial compartment, anti is mediated by the selectin family of cell adhesion molecules. The aim of this study was lo evaluate and characterize the rolling behavior of leukocytes in a model of acute inflammation using a novel soluble selectin ligand directed against P-selectin. Methods: Feline mesenteric postcapillary venules were visualized using intravital microscopy prior to and following exposure to leukotriene C-4 (LTC4) in animals pretreated with vehicle (saline) and the P-selectin antagonistrPSGL-Ig. Results: A concentration of 500 pM LTC4 induced a threefold and sixfold elevation in leukocyte rolling flux and adhesion, respectively, compared to baseline values (p < 0.05). Administration of rPSCL-Ig had no effect on LTC4-induced leukocyte rolling flux but significantly attenuated the increase in the fraction of rolling leukocytes (p < 0.05). In addition, rPSGL-Ig inhibited the LTG(4)-induced reductions in leukocyte rolling velocity (p < 0.001). Finally, LTC4-induced leukocyte adhesion in animals pretreated with rPSGL-Ig was reduced by 60%; compared to vehicle-treated animals GD < 0.05). Conclusions: LTC4 induces leukocyte rolling and adhesion in feline mesenteric venules in a dose-dependent manner. Administration of rPSGL-Ig inhibitsLTC4-induced reductions in leukocyte rolling velocity and attenuates the elevation in the fraction of rolling leukocytes produced by LTC4 stimulation. This suggests that rPSGL-Ig may be used to reduce leukocyte rolling and adhesion, and subsequently attenuate tissue injury during inflammation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:23:54