Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Yeast identification in the clinical microbiology accepted 15 August 2000 laboratory: phenotypical methods
Autore:
Freydiere, AM; Guinet, R; Boiron, P;
Indirizzi:
Hop Antiquaille, Microbiol Lab, F-69321 Lyon 05, France Hop Antiquaille Lyon France 05 e, Microbiol Lab, F-69321 Lyon 05, France Fac Pharm Lyon, Lab Mycol Fondamentale & Appl Biotechnol Ind, F-69373 Lyon, France Fac Pharm Lyon Lyon France F-69373 Biotechnol Ind, F-69373 Lyon, France
Titolo Testata:
MEDICAL MYCOLOGY
fascicolo: 1, volume: 39, anno: 2001,
pagine: 9 - 33
SICI:
1369-3786(200102)39:1<9:YIITCM>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDICALLY IMPORTANT YEASTS; TRANSFORM-INFRARED-SPECTROSCOPY; PYROLYSIS MASS-SPECTROMETRY; CHROMAGAR CANDIDA MEDIUM; 3 COMMERCIAL SYSTEMS; API 20C SYSTEM; GERM-TUBE TEST; RAPID IDENTIFICATION; PRESUMPTIVE IDENTIFICATION; PLUS SYSTEM;
Keywords:
clinical laboratory; enzymatic biochemical methods; physicochemical methods; yeast identification methods;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
131
Recensione:
Indirizzi per estratti:
Indirizzo: Freydiere, AM Hop Antiquaille, Microbiol Lab, F-69321 Lyon 05, France Hop Antiquaille Lyon France 05 ab, F-69321 Lyon 05, France
Citazione:
A.M. Freydiere et al., "Yeast identification in the clinical microbiology accepted 15 August 2000 laboratory: phenotypical methods", MED MYCOL, 39(1), 2001, pp. 9-33

Abstract

Emerging yeast pathogens are favoured by increasing numbers of immunocompromised patients and by certain current medical practices. These yeasts differ in their antifungal drug susceptibilities. and rapid species identification is imperative. A large variety of methods have been developed with the aim of facilitating rapid, accurate yeast identification. Significant recent commercial introductions have included species-specific direct enzymatic colour tests, differential chromogenic isolation plates, direct immunological tests, and enhanced manual and automated biochemical and enzymatic panels. Chromogenic isolation media demonstrate better detection rates of yeastsin mixed cultures than traditional media, and allow the direct identification of Candida albicans by means of colony colour. Comparative evaluation of rapid methods for C. albicans identification, including the germ tube test, shows that chromogenic media may be economically advantageous. Accurate tests for single species include the Bichrolatex Albicans and Krusei Color tests, both immunologically based, as well as the Kernel Rapid Trehalose Assimilation Broth for C. glabrata. Among broad-spectrum tests, the RapID Yeast Plus system gives same-day identification of clinical yeasts, but performance depends on inoculum density and geographic isolate source. The API 20C AUX system is considered a reference method, but newer systems such as Auxacolor and Fungichrom are as accurate and are more convenient. Among automated systems, the ID 32 C strip, the Vitek Yeast Biochemical Card and the Vitek 2 ID-YST system correctly identify >93% of common yeasts, but the ID-YST is the most accurate with uncommon yeasts, including C. dubliniensis. Spectroscopic methods such as Fourier transformed-infrared spectroscopy offer potential advantages for the future. Overall, the advantages of rapid yeast identification methods include relative simplicity and low cost. For allrapid methods, meticulous, standardized multicenter comparisons are neededbefore tests are fully accepted.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 06:34:35