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Titolo:
Development and use of complex probes for DNA fingerprinting the infectious fungi
Autore:
Lockhart, SR; Pujol, C; Joly, S; Soll, DR;
Indirizzi:
Univ Iowa, Dept Biol Sci, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 , Dept Biol Sci, Iowa City, IA 52242 USA
Titolo Testata:
MEDICAL MYCOLOGY
fascicolo: 1, volume: 39, anno: 2001,
pagine: 1 - 8
SICI:
1369-3786(200102)39:1<1:DAUOCP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANDIDA-ALBICANS STRAINS; ASPERGILLUS-FUMIGATUS; GENETIC SIMILARITY; VAGINITIS PATIENTS; CA3; DUBLINIENSIS; IDENTIFICATION; HYBRIDIZATION; MAINTENANCE; SEQUENCE;
Keywords:
Candida; complex probe; DNA fingerprinting; infectious fungi;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Lockhart, SR Univ Iowa, Dept Biol Sci, 314 Biol Bldg E, Iowa City, IA 52242 USA Univ Iowa 314 Biol Bldg E Iowa City IA USA 52242 IA 52242 USA
Citazione:
S.R. Lockhart et al., "Development and use of complex probes for DNA fingerprinting the infectious fungi", MED MYCOL, 39(1), 2001, pp. 1-8

Abstract

A variety of methods have emerged for genetic fingerprinting the infectious fungi. One of the most versatile is Southern blot hybridization with species-specific complex DNA probes that include sequences that identify hypervariable, moderately variable and invariant genomic sequences. These probes assess genetic relatedness at all the necessary levels including identical,highly related but non-identical, moderately related and unrelated. Methods are described for cloning complex probes, characterizing them and verifying their effectiveness at the different levels of resolution. The complex probes that have been developed for Candida albicans, C. glabrata, C. dubliniensis, C. tropicalis, C. parapsilosis and Aspergillus fumigatus are described and discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 11:09:29