Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Molecular cloning and characterization of two isoforms of trypsinogen fromanchovy pyloric ceca
Autore:
Ahsan, MN; Funabara, D; Watabe, S;
Indirizzi:
Univ Tokyo, Grad Sch Agr & Life Sci, Lab Aquat Mol Biol & Biotechnol, Tokyo, Japan Univ Tokyo Tokyo Japan i, Lab Aquat Mol Biol & Biotechnol, Tokyo, Japan
Titolo Testata:
MARINE BIOTECHNOLOGY
fascicolo: 1, volume: 3, anno: 2001,
pagine: 80 - 90
SICI:
1436-2228(200101/02)3:1<80:MCACOT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
COD GADUS-MORHUA; ATLANTIC COD; CONVERTING TRYPSIN; SERINE PROTEASES; SUBSTRATE-SPECIFICITY; GREENLAND COD; CHYMOTRYPSIN; PURIFICATION; PROTEIN; SEQUENCE;
Keywords:
anchovy; serine protease; trypsinogen; trypsin; structure; cDNA cloning;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Watabe, S Univ Tokyo, Grad Sch Agr & Life Sci, Lab Aquat Mol Biol & Biotechnol, Tokyo, Japan Univ Tokyo Tokyo Japan at Mol Biol & Biotechnol, Tokyo, Japan
Citazione:
M.N. Ahsan et al., "Molecular cloning and characterization of two isoforms of trypsinogen fromanchovy pyloric ceca", MAR BIOTEC, 3(1), 2001, pp. 80-90

Abstract

Complementary DNA clones encoding two isoforms of trypsinogen were isolated from the pyloric ceca of anchovy by rapid amplification of cDNA ends (RACE). Nucleotide sequences of isolated clones encoded, in addition to characteristic signal and activation peptides, two isoforms of trypsin containing 220 and 221 amino acid residues. Both enzymes contained the catalytic triadof a serine protease, together with the residues determining substrate specificity. The anchovy trypsins showed a high amino acid identity of about 80% to those of other fish species. Southern blot analysis with a probe cross-reactive to both isoforms showed a complex genomic pattern. Northern blotanalysis with the same probe revealed the highest expression of meassengerRNA in the pyloric ceca. Structural parameters possibly involved in highercatalytic properties of fish trypsin were examined by three-dimensional modeling, which included deletion in the autolysis loop, lack of Tyr-151 at the entrance of the S1 pocket, and distribution of charged residues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:40:05