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Titolo:
Fibroblast growth factor inhibits expression of neural markers in culturesof zebrafish early embryo cells
Autore:
Singh, NN; Fischer, K; Hedstrom, O; Barnes, DW;
Indirizzi:
Natl Stem Cell Resource, Amer Type Culture Collect, Manassas, VA 20110 USANatl Stem Cell Resource Manassas VA USA 20110 ect, Manassas, VA 20110 USA Oregon State Univ, Coll Vet Med, Corvallis, OR 97331 USA Oregon State Univ Corvallis OR USA 97331 Vet Med, Corvallis, OR 97331 USA
Titolo Testata:
MARINE BIOTECHNOLOGY
fascicolo: 1, volume: 3, anno: 2001,
pagine: 27 - 35
SICI:
1436-2228(200101/02)3:1<27:FGFIEO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MIDBRAIN-HINDBRAIN BOUNDARY; PRIMORDIAL GERM-CELLS; STEM-CELLS; GENE; XENOPUS; TRANSCRIPTION; SPECIFICATION; DERIVATION; PATHWAY; FAMILY;
Keywords:
fibroblast growth factor; development; neurogenesis; zebrafish; stem cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Barnes, DW Natl Stem Cell Resource, Amer Type Culture Collect, 10801 Univ Blvd, Manassas, VA 20110 USA Natl Stem Cell Resource 10801 Univ Blvd Manassas VA USA 20110 A
Citazione:
N.N. Singh et al., "Fibroblast growth factor inhibits expression of neural markers in culturesof zebrafish early embryo cells", MAR BIOTEC, 3(1), 2001, pp. 27-35

Abstract

Basic fibroblast growth factor (FGF) regulation of developmental markers in cell cultures derived from early zebrafish embryos was examined with the goal of in vitro culture of zebrafish embryonic stem cells and gaining an understanding of extracellular influences on early embryonic development. Markers were stem/primordial germ cell markers pou-2 and vas, neural markers zp-50, pax[zf-a], en-3, and wnt-1, and mesodermal markers gsc and myoD. Previously we had shown that FGF prevents the development of zebrafish pigmentcells in vitro. In our culture system, FGF reduced expression of neural-specific markers, possibly implicating the FGF family in suppression of earlyneural cell development. Exposure to FGF for 24 hours at the time of seeding the cells was sufficient to suppress neural marker expression for a subsequent 4 days of culture, while absence of FGF for the first 24 hours of culture nullified the effect of FGF added subsequently. FGF predictably increased expression of gsc and myoD. Vas expression was unaffected, while pou-2expression decreased with time in culture in the presence or absence of FGF. However, in situ hybridization identified a subpopulation of cells expressing pou-2, suggesting the possible continued existence of undifferentiated stem cells in the cultures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:00:15