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Titolo:
DIFFERENCES IN RESPONSES OF INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR-ALPHA PRODUCTION AND SECRETION TO CYCLOSPORINE-A AND ULTRAVIOLET B-IRRADIATION BY NORMAL AND TRANSFORMED KERATINOCYTE CULTURES
Autore:
MARIONNET AV; CHARDONNET Y; VIAC J; SCHMITT D;
Indirizzi:
HOP EDOUARD HERRIOT,INSERM U346,PAVILLON R F-69437 LYON 03 FRANCE HOP EDOUARD HERRIOT,INSERM U346 F-69437 LYON 03 FRANCE
Titolo Testata:
Experimental dermatology
fascicolo: 1, volume: 6, anno: 1997,
pagine: 22 - 28
SICI:
0906-6705(1997)6:1<22:DIROIA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADHESION MOLECULE-1 EXPRESSION; MESSENGER-RNA EXPRESSION; CARCINOMA CELL-LINES; RECEPTOR ANTAGONIST; CYTOKINE PRODUCTION; HUMAN SKIN; ICAM-1 EXPRESSION; GENE-EXPRESSION; IFN-GAMMA; TNF-ALPHA;
Keywords:
NORMAL HUMAN KERATINOCYTES; HPV-TRANSFORMED KERATINOCYTES; IL-1 EXPRESSION; TNF-ALPHA EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
A.V. Marionnet et al., "DIFFERENCES IN RESPONSES OF INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR-ALPHA PRODUCTION AND SECRETION TO CYCLOSPORINE-A AND ULTRAVIOLET B-IRRADIATION BY NORMAL AND TRANSFORMED KERATINOCYTE CULTURES", Experimental dermatology, 6(1), 1997, pp. 22-28

Abstract

Among epidermal cytokines, IL-1 and TNF alpha are involved in inflammatory skin reactions and suspected of modulation by immunosuppressive treatment (e.g., cyclosporin A, CsA) or UVB-irradiation, 2 mediators probably being involved in epithelial carcinogenesis, We evaluated the effects of 8 mu g/ml CsA and 100 J/m(2) UVB-irradiation of the production and secretion of IL-1 and TNF alpha on normal hunan epidermal keratinocytes (NHK) and epidermal keratinocyte cell lines either spontaneously transformed (HaCaT) or transformed by human papillomavirus (HPV) type 16 or 18 (EK16 and EK18), by using ELISA test. Normal and immortalized keratinocytes constitutively produced and re]eased IL-1 alpha, IL-1 beta and IL-2 receptor antagonist (IL-1RA) but IL-1 synthesis by NHK was significantly higher than by cell lines. All the cells spontaneously excreted low amounts of TNF alpha., Different responses to treatments were evidenced between NHK and cell lines. CsA modified significantly the production and secretion of IL1 in most cells whereas slightchanges were observed with TNF alpha secretion, UVB irradiation had no effect on the intracellular IL1 pool of any cells but increased the release of IL1 and TNF alpha, The association CsA-UVB did not result in additive effects on synthesis and secretion of IL1; the release of TNF alpha by the cells remained pear except for EK18 cells. Taken together, these results show that, in immortalized keratinocytes, the IL-1 and TNF alpha expression was differently affected by treatments with CsA and/or UVB-irradiation as compared to NHK. In addition, spontaneously transformed keratinocytes, HaCaT, reacted differently from HPV-transformed keratinocytes, EK16 and EK18.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 19:24:23