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Titolo:
Growth factors regulation of rabbit sodium-dependent neutral amino acid transporter ATB(0) and oligopeptide transporter 1 mRNAs expression after enterectomy
Autore:
Avissar, NE; Ziegler, TR; Wang, HT; Gu, LH; Miller, JNH; Iannoli, P; Leibach, FH; Ganapathy, V; Sax, HC;
Indirizzi:
Univ Rochester, Med Ctr, Dept Surg, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 Dept Surg, Rochester, NY 14642 USA Emory Univ, Sch Med, Dept Med, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 Sch Med, Dept Med, Atlanta, GA 30322 USA Med Coll Georgia, Dept Biochem & Mol Biol, Augsburg, Germany Med Coll Georgia Augsburg Germany Biochem & Mol Biol, Augsburg, Germany
Titolo Testata:
JOURNAL OF PARENTERAL AND ENTERAL NUTRITION
fascicolo: 2, volume: 25, anno: 2001,
pagine: 65 - 72
SICI:
0148-6071(200103/04)25:2<65:GFRORS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRUSH-BORDER MEMBRANE; SHORT-BOWEL SYNDROME; HUMAN SMALL-INTESTINE; L-LEUCINE TRANSPORT; RAT SMALL-INTESTINE; FUNCTIONAL-CHARACTERIZATION; PEPTIDE TRANSPORTER; GLUCOSE-TRANSPORT; BROAD-SCOPE; SYSTEM-B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Avissar, NE Univ Rochester, Med Ctr, Dept Surg, 601 Elmwood Ave, Rochester, NY 14642 USA Univ Rochester 601 Elmwood Ave Rochester NY USA 14642 4642 USA
Citazione:
N.E. Avissar et al., "Growth factors regulation of rabbit sodium-dependent neutral amino acid transporter ATB(0) and oligopeptide transporter 1 mRNAs expression after enterectomy", J PARENT EN, 25(2), 2001, pp. 65-72

Abstract

Background: Sucessful intestinal adaptation after massive enterectomy is dependent on increased efficiency of nutrient transport. However, midgut resection (MGR) in rabbits induces an initial decrease in sodium-dependent brush border neutral amino acid transport, whereas parenteral epidermal growthfactor (EGF) and growth hormone (GH) reverse this downregulation. We investigated intestinal amino acid transporter B-0 (ATB(0)) and oligopeptide transporter 1 (PEPT 1) mRNA expression after resection and in response to EGF and/or GH. Methods: Rabbits underwent anesthesia alone (control) or proximal, midgut, and distal resections. Full-thickness intestine was harvested from all groups on postoperative day (POD) 7, and on POD 14 from control and MGR rabbits. A second group of MGR rabbits received EGF and/or GH for 7 days, beginning 7 days after resection. ATB(0) and PEPT I mRNA levels were determined by Northern blot analysis. Results: In control animals, ileal ATB(0) mRNA abundance was three times higher than jejunal mRNA, whereas PEPT I mRNA expression was similar. By 7 and 14 days after MGR, jejunal ATB(0) mRNAabundance was decreased by 50% vs control jejunum. A 50% decrease in jejunal PEPT 1 message was delayed until 14 days after MGR. Treatment with EGF plus GH did not alter ATB(0) mRNA expression but doubled PEPT 1 mRNA in the jejunum. Conclusion: The site of resection, time postresection, and growth factors treatment differentially influence ATB(0) and PEPT 1 mRNA expression. Enhanced sodium dependent brush border neutral amino acid transport withGH plus EGF administration is independent of increased ATB(0) mRNA expression in rabbit small intestine after enterectomy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 09:16:42