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Titolo:
Monoclonal antibody against brain natriuretic peptide and characterizationof brain natriuretic peptide-transgenic mice
Autore:
Nakagawa, M; Tanaka, I; Mukoyama, M; Suga, S; Ogawa, Y; Tamura, N; Ishibashi, R; Goto, M; Nakagawa, O; Sugawara, A; Nakao, K;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Med & Clin Sci, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 Clin Sci, Sakyo Ku, Kyoto 6068507, Japan
Titolo Testata:
JOURNAL OF HYPERTENSION
fascicolo: 3, volume: 19, anno: 2001,
pagine: 475 - 483
SICI:
0263-6352(200103)19:3<475:MAABNP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONGESTIVE-HEART-FAILURE; ACUTE MYOCARDIAL-INFARCTION; CARDIAC HORMONE; HYPERTENSIVE RATS; MOLECULAR-CLONING; DOWN-REGULATION; POLYPEPTIDE; RECEPTOR; EXPRESSION; GENE;
Keywords:
brain natriuretic peptide; monoclonal antibody; radioimmunoassay; neutralization; transgenic mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, I Kyoto Univ, Grad Sch Med, Dept Med & Clin Sci, Sakyo Ku, 54 Shogoin Kawahara-cho, Kyoto 6068507, Japan Kyoto Univ 54 Shogoin Kawahara-cho Kyoto Japan 6068507 7, Japan
Citazione:
M. Nakagawa et al., "Monoclonal antibody against brain natriuretic peptide and characterizationof brain natriuretic peptide-transgenic mice", J HYPERTENS, 19(3), 2001, pp. 475-483

Abstract

Objective Brain natriuretic peptide (BNP) is a ventricular hormone with natriuretic, diuretic and vasodilatory actions, Acute infusion of BNP reducescardiac pre- and after-load in healthy and diseased subjects, but its long-term therapeutic usefulness remains unclear,Design: We prepared a monoclonal antibody specific to mouse BNP, and characterized transgenic mice overexpressing BNP in the liver (BNP-Tg mice) as amodel of its chronic overproduction. Methods: Radioimmunoassay and neutralization experiments using the monoclonal antibody, KY-mBNIP-I, were performed in BNP-Tg mice in conjunction withexaminations of blood pressure (BP) and other markers for body fluid homeostasis,Results: We developed highly sensitive radioimmunoassay to mouse BNP. In BNP-Tg mice, the plasma BNP concentration increased more than 100-fold, while ventricular BNP concentration did not alter, suggesting that ventricular BNP production was not down-regulated in BNP-Tg mice. The BNP concentrationin the kidneys was 10-fold higher than nontransgenic (nonTg) littermates, accompanied with marked reduction in the atrial natriuretic peptide (ANP) concentration, that may be due to binding of circulating BNP to the natriuretic peptide receptors, BNP-Tg mice showed significantly low arterial BP, and a bolus intraperitoneal administration of KY-mBNIP-I completely abolishedenhanced cGMP excretion in the urine and significantly increased the systolic BP,Conclusion: These results suggested that biological actions of BNP last and reduce cardiac overload in its longterm overproduction in the transgenic mouse model. I Hypertens 19:475-483 (C) 2001 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 08:33:49