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Titolo:
Chloroplast precursor proteins compete to form early import intermediates in isolated pea chloroplasts
Autore:
Row, PE; Gray, JC;
Indirizzi:
Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England Univ CambridgeCambridge England CB2 3EA Sci, Cambridge CB2 3EA, England Univ Cambridge, Cambridge Ctr Mol Recognit, Cambridge CB2 3EA, England Univ Cambridge Cambridge England CB2 3EA nit, Cambridge CB2 3EA, England
Titolo Testata:
JOURNAL OF EXPERIMENTAL BOTANY
fascicolo: 354, volume: 52, anno: 2001,
pagine: 47 - 56
SICI:
0022-0957(200101)52:354<47:CPPCTF>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
INNER ENVELOPE MEMBRANE; TRANSIT PEPTIDE; OUTER ENVELOPE; BINDING PROTEIN; ARABIDOPSIS-THALIANA; THYLAKOID MEMBRANE; ESCHERICHIA-COLI; RNA-POLYMERASE; SMALL SUBUNIT; CLONED GENES;
Keywords:
chloroplast protein import; LHCP; phosphate translocator; precursor protein; Rieske protein; Rubisco; translocation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Gray, JC Univ Cambridge, Dept Plant Sci, Downing St, Cambridge CB2 3EA, England Univ Cambridge Downing St Cambridge England CB2 3EA 3EA, England
Citazione:
P.E. Row e J.C. Gray, "Chloroplast precursor proteins compete to form early import intermediates in isolated pea chloroplasts", J EXP BOT, 52(354), 2001, pp. 47-56

Abstract

In order to ascertain whether there is one site for the import of precursor proteins into chloroplasts or whether different precursor proteins are imported via different import machineries, chloroplasts were incubated with large quantities of the precursor of the 33 kDa subunit of the oxygen-evolving complex (pOE33) or the precursor of the light-harvesting chlorophyll a/b-binding protein (pLHCP) and tested for their ability to import a wide range of other chloroplast precursor proteins. Both pOE33 and pLHCP competed for import into chloroplasts with precursors of the stromally-targeted small subunit of Rubisco (pSSu), ferredoxin NADP(+) reductase (pFNR) and porphobilinogen deaminase; the thylakoid membrane proteins LHCP and the Rieske iron-sulphur protein (pRieske protein); ferrochelatase and the gamma subunit ofthe ATP synthase (which are both associated with the thylakoid membrane); the thylakoid lumenal protein plastocyanin and the phosphate translocator, an integral membrane protein of the inner envelope. The concentrations of pOE33 or pLHCP required to cause half-maximal inhibition of import ranged between 0.2 and 4.9 muM. These results indicate that all of these proteins are imported into the chloroplast by a common import machinery. Incubation ofchloroplasts with pOE33 inhibited the formation of early import intermediates of pSSu, pFNR and pRieske protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 18:17:05