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Titolo:
Phosphatidylinositol-specific phospholipase C (PI-PLC) cleavage of GPI-anchored surface molecules of Trypanosoma cruzi triggers in vitro morphological reorganization of trypomastigotes
Autore:
Mortara, RA; Minelli, LMS; Vandekerchove, F; Nussenzweig, V; Ramalho-Pinto, FJ;
Indirizzi:
Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, Brazil Univ Fed Sao Paulo Sao Paulo Brazil BR-04023062 BC3062 Sao Paulo, Brazil Univ Fed Sao Paulo, Escola Paulista Med, Ctr Microscopia Eletron, BR-04023062 Sao Paulo, Brazil Univ Fed Sao Paulo Sao Paulo Brazil BR-04023062 BC3062 Sao Paulo, Brazil Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Paasitol Microbiol & Imunol, BR-14049900 Ribeirao Preto, SP, Brazil Univ Sao Paulo Ribeirao Preto SP Brazil BR-14049900 BCo Preto, SP, Brazil NYU Med Ctr, Dept Pathol, Div Immunol, New York, NY 10016 USA NYU Med CtrNew York NY USA 10016 ol, Div Immunol, New York, NY 10016 USA
Titolo Testata:
JOURNAL OF EUKARYOTIC MICROBIOLOGY
fascicolo: 1, volume: 48, anno: 2001,
pagine: 27 - 37
SICI:
1066-5234(200101/02)48:1<27:PPC(CO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; HOST-CELL INVASION; MAMMALIAN-CELLS; SIGNAL-TRANSDUCTION; BRUCEI; DIFFERENTIATION; CYTOSKELETON; AMASTIGOTES; PROTEIN; MEMBRANE;
Keywords:
confocal microscopy; flagellum; GPI; PI-PLC; protozoa; Trypanosoma cruzi; trypomastigote;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Mortara, RA Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, Brazil Univ Fed Sao Paulo Sao Paulo Brazil BR-04023062 BClo, Brazil
Citazione:
R.A. Mortara et al., "Phosphatidylinositol-specific phospholipase C (PI-PLC) cleavage of GPI-anchored surface molecules of Trypanosoma cruzi triggers in vitro morphological reorganization of trypomastigotes", J EUKAR MIC, 48(1), 2001, pp. 27-37

Abstract

Trypanosoma cruzi trypomastigotes treated with phosphatidylinositol-specific phospholipase C (PI-PLC) in vitro are rapidly induced to differentiate into round forms. Using confocal microscopy, we were able to show that trypomastigotes treated with PI-PLC initiate the process of flagellum remodelingby 30 sec after contact with the enzyme and amastigote-like forms are detected as early as 10 min after PI-PLC treatment. Scanning and transmission electron microscopy indicate that trypomastigotes undergo a previously undescribed process of flagellum circularization and internalization. Analysis of the flagellar complex with monoclonal antibody 4D9 shows heterogeneous labeling among the parasites, suggesting a remodeling of these molecules. After PI-PLC treatment, parasites rapidly lose the surface marker Ssp-3 and 24h post-treatment they begin to exhibit a circular nucleus and a rod-shapedkinetoplast. By flow cytometry analysis and confocal microscopy, the Ssp-4amastigote-specific epitope can be detected on the parasite surface. This indicates that thr release of trypomastigote GPI-anchored molecules by exogenous PI-PLC in vitro can trigger morphological changes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:19:23