Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Stimulation of plasminogen activator activity and matrix metalloproteinases of human dental pulp-derived cells by tumor necrosis factor-alpha
Autore:
Ueda, I; Matsushima, K;
Indirizzi:
Nihon Univ, Sch Dent, Dept Endodont, Matsudo, Chiba 2718587, Japan Nihon Univ Matsudo Chiba Japan 2718587 ont, Matsudo, Chiba 2718587, Japan
Titolo Testata:
JOURNAL OF ENDODONTICS
fascicolo: 3, volume: 27, anno: 2001,
pagine: 175 - 179
SICI:
0099-2399(200103)27:3<175:SOPAAA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PURIFICATION; CYTOKINES; INHIBITOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Matsushima, K Nihon Univ, Sch Dent, Dept Endodont, 2-870-1 Sakaecho Nishi,Matsudo, Chiba 2718587, Japan Nihon Univ 2-870-1 Sakaecho Nishi Matsudo Chiba Japan 2718587
Citazione:
I. Ueda e K. Matsushima, "Stimulation of plasminogen activator activity and matrix metalloproteinases of human dental pulp-derived cells by tumor necrosis factor-alpha", J ENDODONT, 27(3), 2001, pp. 175-179

Abstract

The effects of tumor necrosis factor (TNF)-alpha on the plasminogen activator (PA)/plasmin system in human dental pulp (HDP) cells were examined, TNF-alpha treatment induced a significantly high level of PA activity in the conditioned medium of HDP cells in a time- and dose-dependent manner, compared with untreated control cells. Western-blot analysis revealed that tissuetype (t)PA protein in conditioned medium was increased by TNF-alpha when compared with control medium. Furthermore the tPA mRNA level had increased in HDP cells treated with TNF-alpha, as determined by reverse transcription-polymerase chain reaction, but urokinase PA and PA inhibitor-1 mRNA levels did not increase. We examined the effects of TNF-alpha against activities of matrix metalloproteinases (MMPs) using zymography, TNF-alpha stimulated MMP-2 activity in conditioned medium and stimulated MMP-9 activity with addition of plasminogen into conditioned medium. The present results suggested that TNF-alpha stimulates PA activity via an enhancement of tPA gene expression in HDP cells and MMP-2 activity, and further that tPA-activated TNF-alpha stimulated MMP-9.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 11:53:38